Expression And Significance Of BRAF V600E In Papillary Thyroid Carcinoma In Inner Mongolia

Objective:BRAF V600 E mutation is the most common molecular marker in papillary thyroid carcinoma(PTC).BRAF V600 E mutation plays an important role in the occurrence and development of PTC.Although many studies have reported the relationship between BRAF V600 E mutation and clinicopathological characteristics of PTC,it is still controversial to some extent.Many studies have shown that the mutation rate and correlation of BRAF V600 E mutation in different countries,different regions and different nationalities are different,but there is no report on the correlation of BRAF V600 E mutation in Mongolian and Han PTC patients in Inner Mongolia.In this study,the mutation rate and distribution of BRAF V600 E in Mongolian and Han PTC patients in Inner Mongolia were determined.Then,the difference of BRAF V600 E mutation rate between Mongolian and Han PTC patients in Inner Mongolia,the relationship between BRAF V600 E mutation and clinicopathological characteristics of Mongolian PTC patients in Inner Mongolia,and the influencing factors of central lymph node metastasis in Mongolian PTC patients in Inner Mongolia were evaluated.Method:Retrospective analysis was performed on patients diagnosed with PTC by surgical excision and pathology in the Department of Thyroid and Breast Surgery,our university from January 2017 to December 2022.According to the screening criteria,60 Han patients with PTC and 60 Mongolian patients with PTC were screened out.Clinicopathological data and archive paraffin tissue of all patients were collected.BRAF V600 E mutation was detected by second generation sequencing.Chi-square test was used to analyze the difference of BRAF V600 E mutation rate between Mongolian and Han PTC patients in Inner Mongolia and the relationship between BRAF V600 E mutation and clinicopathological characteristics of Mongolian PTC patients in Inner Mongolia.Chi-square test and binary Logistic regression were used to analyze the influencing factors of central lymph node metastasis in Mongolian PTC patients in Inner Mongolia.Results:(1)BRAF V600 E mutation was found in 45 of 60 Mongolian PTC patients,and the mutation incidence was 75.0%.BRAF V600 E mutation was found in 32 of 60 Han patients with PTC,with an incidence of 53.3%.The difference of mutation rate of BRAF V600 E between Mongolian PTC patients and Han PTC patients in Inner Mongolia was statistically significant(P<0.05).The mutation rate of BRAF V600 E in Mongolian PTC patients was significantly higher than that in Han PTC patients.(2)Chi-square test showed that BRAF V600 E mutation and Mongolian PTC patients in Inner Mongolia were significantly different in gender,age,central lymph node metastasis,whether Hashimoto was associated with PTC,and tunic invasion(P<0.05),and there were no significant differences in tumor diameter,number of lesions,nodules or calcification(P>0.05).(3)Chi-square test showed that there were statistically significant differences between lymph node metastasis,nodules calcification and BRAF V600 E mutation in Mongolian PTC patients in Inner Mongolia(P<0.05).The presence or not of nodules calcification and BRAF V600 E mutation were included in the binary Logistic regression equation,and the results showed that the presence or not of nodules calcification had a statistically significant effect on central lymph node metastasis in Mongolian PTC patients in Inner Mongolia(OR=3.85,95%CI 1.16-12.80,P<0.05).The effect of BRAF V600 E mutation on central lymph node metastasis in Mongolian PTC patients in Inner Mongolia was statistically significant(OR=4.84,95%CI 1.28-18.33,P<0.05).Conclusion:(1)The mutation rate of BRAF V600 E in Mongolian PTC patients is different from that in Han PTC patients in Inner Mongolia.The mutation rate of BRAF V600 E in Mongolian PTC patients is significantly higher than that in Han PTC patients,and this difference may be related to various factors.(2)BRAF V600 E mutation can improve the diagnosis rate of PTC patients of Mongolian nationality in Inner Mongolia,and provide certain reference for the formulation of surgery,follow-up and prognosis evaluation of patients.(3)Calcification of nodules and BRAF V600 E mutation can effectively predict whether the central lymph node metastasis occurs in Mongolian PTC patients in Inner Mongolia.