| Objective: Based on the Mongolian medical literature records and previous research,this study proposed the hypothesis that Mongolian moxibustion may have the effect of regulating gastrointestinal hormone secretion and promoting gastrointestinal motility in FD model rats,thereby improving the function of the enteric nervous system,and clarified the influence of Mongolian moxibustion on gastric motility in FD model rats and the complex mechanism of promoting intestinal nervous function in FD model rats.Methods: 43 SPF grade SD male rats were randomly selected as a blank group using the SPSS random sampling procedure,and the other 32 rats were selected as the FD group.The FD group rats were modeled using the "irregular diet+tail clipping stimulation" method.A total of 28 days of modeling,the blank group of rats without any treatment.The success of modeling was verified through general status,body weight,average food consumption,average water consumption,HE staining of 3 rats were used to verify the success of modeling.After confirming the success of modeling,the remaining 30 rats in the FD group were randomly divided into four groups: western medicine group,moxibustion group,model group,and previous blank group,with 10 rats in each group.The blank group was treated with mosapride without treatment,while the moxibustion group was treated with moxibustion at Heyi,Huoshuai,and Stomach points.The model group was fixed as the moxibustion group without treatment.A total of 12 days of treatment.After treatment,the gastric emptying rate and small intestine propulsion rate were measured,MTL and VIP levels in serum were measured by ELISA,and S100 was examined by immunofluorescence staining β Positive distribution and semi quantitative analysis.Immunohistochemical staining was used to observe the distribution and semi quantitative analysis of GDNF positive staining.Detection of S100 using the Western Blot method β、 The relative expression levels of GFAP,PGP9.5,GDNF,P-MEK,and p-ERK1/2 proteins.Result:1.Compared with the blank group,the measurement results of gastric emptying rate and intestinal propulsion rate in the model group showed a significant difference(P<0.01);Compared with the model group,there was a significant difference in gastric emptying rate between the moxibustion group and the western medicine group(P<0.01);There was a significant difference in the small intestine propulsion rate between the model group and the blank group(P<0.01).Compared with the model group,there was a significant difference in the intestinal propulsion rate between the moxibustion group and the western medicine group(P<0.01).2.The serum motilin and vasoactive intestinal peptide levels in the model group were significantly different from those in the blank group(P<0.01);Compared with the model group,there was a significant difference in serum motilin concentration between the moxibustion group and the western medicine group(P<0.01);Compared with the blank group,there was a significant difference in serum vasoactive intestinal peptide concentration in the model group(P<0.05).Compared with the model group,there was a significant difference in serum vasoactive intestinal peptide concentration in the moxibustion group(P<0.05).Compared with the model group,there was no significant difference in serum vasoactive intestinal peptide concentration in the western medicine group(P>0.05).3.s100 in gastric antrum tissue βImmunofluorescence staining results: Immunofluorescence staining of gastric antrum tissue showed that positive staining was mainly distributed in the mucosal layer and intrinsic basal layer,indicating stronger staining and fluorescence in the model.According to semi quantitative analysis,there was a significant difference between the model group and the blank group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01).4.WB detection of s100 in gastric antrum tissue β 、 Results of relative expression levels of GFAP and PGP9.5 proteins,gastric antrum tissue s100 β The results of WB showed that there was a significant difference between the model group and the blank group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01).The WB results of the relative expression of GFAP protein in gastric antrum tissue showed a significant difference compared to the blank group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01);The WB results of the relative expression of PGP9.5 protein in gastric antrum tissue showed a significant difference compared to the blank group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01).5.Immunohistochemical staining results of GDNF in gastric antrum tissue: Immunohistochemical staining of gastric antrum tissue can be seen.Positive staining is mainly distributed in the intrinsic muscle layer of gastric antrum tissue,and the positive staining in the model group is deeper and more obvious.Semi quantitative analysis results show that there is a significant difference(P<0.05)compared to the blank group in the model group.Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.05).6.WB detection of the relative expression of GDNF,p-MEK,and p-ERK1/2 in gastric antrum tissue showed a significant difference compared to the blank group compared to the model group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01);The WB results of the relative expression of p-MEK protein in gastric antrum tissue showed a significant difference compared to the blank group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01);The WB results of the relative expression of p-ERK1/2 protein in gastric antrum tissue showed a significant difference compared to the blank group in the model group(P<0.01).Compared with the model group,there was a significant difference between the moxibustion group and the western medicine group(P<0.01).Conclusion:1.Mongolian medicine moxibustion has the effect of promoting gastric emptying and intestinal propulsion in FD model rats,and its mechanism may be related to promoting MTL secretion and inhibiting VIP secretion.2.Mongolian medicine moxibustion can affect gastrointestinal motility and promote intestinal nerve function in FD model rats,and its mechanism may be related to its inhibition of s100 β、 GFAP,inhibition of p-MEK,p-ERK1/2,and upregulation of PGP9.5 are related to the restoration of gastrointestinal motility. |
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