Correlation Study Of MTNR1B Gene Polymorphisms On Adverse Neonatal Birth Outcomes

Objective To investigate the MTNR1 B gene polymorphism of mothers and explore the effects of maternal MTNR1 B gene polymorphism on adverse birth outcomes of newborns(mainly including premature delivery,low birth weight,hyperbilirubinemia,macrosomia)Methods Based on the case-control study method,a cluster sampling of mothers and children who received late pregnancy examination in Gansu Maternal and Child Health Hospital from March 2021 to October 2021 were included in the prospective cohort,and 180 children diagnosed with premature birth were random Ly selected as the case group.At the same time,maternal BMI was used as the matching condition to conduct 1:1 matching healthy newborns as the control group.The general family status(sociodemographic characteristics and maternity history,etc.)and mother’s living habits of newborns were investigated.The genomic DNA of maternal peripheral blood was collected and detected.The gene polymorphisms of MTNRIB rs10830963,rs3781637 and rs3781638 were detected by im LDR technique.Univariate analysis and multivariate logistic regression were used to analyze the correlation between allele frequency,co-dominance and preterm birth.A case-control study was carried out in 133 infants diagnosed with low birth weight,118 neonates with hyperbilirubinemia,88 macros and 180 healthy neonates.The polymorphism of MTNRIB rs10830963,rs3781637,rs3781638 gene was associated with low birth weight infants,hyperbilirubinemia and macrosomia infants.To investigate the association between maternal MTNR1 B gene polymorphism and preterm birth,low birth weight,hyperbilirubinemia and macrosomia.Results1.The association between the polymorphism of MTNR1 B gene and preterm birth MTNRIB rs3781637(C > T)CC,CT and TT genotype frequencies were statistically different between the preterm birth group and the control group(P < 0.05).After adjusting forconfounding factors,CT-TT was a protective factor for preterm birth(OR=0.169,95%CI:0.054～0.530,P=0.002 < 0.05)compared with CC genotype under the dominant gene model.MTNRIB rs3781637(C > T)TT was a protective factor for preterm birth compared with CC-CT genotype in recessive gene mode(OR: 0.433,95%CI:0.244～0.766,P=0.004 <0.05),there was no significant difference in the distribution of residual gene frequencies between the preterm birth group and the control group under other models(P > 0.05).2.The association between MTNR1 B gene polymorphism and low birth weight infants MTNRIB rs3781637(C > T)CC,CT and TT genotype frequency distribution in low birth weight group and control group was statistically significant(P < 0.05).After adjusting for confounding factors,MTNRIB rs3781637(C > T)was a protective factor for low birth weight compared with CC genotype under dominant gene inheritance model(OR=0.098,95%CI:0.029-0.325,P < 0.001 < 0.05).MTNRIB rs3781637(C > T)in recessive gene mode,TT was a protective factor for low birth weight compared with CC-CT genotype(OR: 0.248,95%CI:0.128～0.477,P < 0.001 < 0.05).There was no significant difference in the distribution of residual gene frequencies between the preterm delivery group and the control group under other models(P > 0.05).3.The association between MTNR1 B gene polymorphism and hyperbilirubinemia(C >T)The distribution of CC,CT and TT genotype frequencies in hyperbilirubinemia group and control group was statistically significant(P < 0.05).After adjusting for confounding factors,MTNRIB rs3781637(C > T)was a protective factor for low birth weight in newborn compared with CC genotype under dominant gene inheritance model(OR=0.206,95%CI:0.056-0.760,P < 0.018 < 0.05).There was no significant difference in the distribution of residual gene frequencies between hyperbilirubinemia group and the control group under other models(P > 0.05).4.MTNR1 B gene polymorphism and macrosomia codominant,dominant,recessive and superdominant models of MTNRIB rs10830963(G > C),MTNRIB rs3781637(C > T)and MTNRIB rs3781638(C > T)showed no statistical difference between the macroso group and control groupmeaning(P > 0.05).ConclusionsThe polymorphism of MTNR1 B gene rs3781637 was associated with preterm birth,low birth weight and hyperbilirubinemia in neonates in allele or genotype effect models.