Study Of Spectrometer System For Fluorescence Immunoassay Analyzer

As a new medical detection method,fluorescence immunoassay relies on the fluorescence reaction mechanism of the substance to analyze and detect the reagents to be detected,which has the advantages of fast and real-time compared with the traditional turbidimetric method and colorimetric method.However,most of the fluorescence immunoassay analyzers currently used in China use single-channel detection,and there are limitations for multi-element analysis of the same reagent to be detected,which requires multiple testing.Therefore,in this paper,a spectrometer system is designed to solve the above problems by analyzing the measurement principle and technology of fluorescence immunoassay analyzer and using the grating spectroscopy principle.The main work of this paper is as follows:1)This paper analyzes the technical characteristics of immunoassay analyzers,and proposes a grating dispersive spectrometer system based on grating spectroscopy technology as an optical scheme for the detection system of fluorescence immunoassay analyzers based on the fluorescence effect principle for the shortcomings of traditional immunoassay analyzers,and verifies the feasibility of the scheme through simulation analysis of the spectrometer system.2)This paper analyzes the advantages and disadvantages of various reflectance spectrometer system structures,and determines the Czerny-Turner structure as the optical system for spectral imaging of fluorescence immunoassay analyzer according to the requirements of the fluorescence immunoassay analyzer for form factor,resolution of fluorescence spectrum,and spectral range.3)This paper,we simulated the spectral imaging system by building an optical system model and evaluated various aberrations existing in the system.For the problem of monochromatic aberrations caused by off-axis mirrors,a correction scheme using aspherical lenses as correction mirrors is proposed to effectively reduce the aberrations of the Czerny-Turner structure.Through simulation,the feasibility of the scheme is verified,and it can effectively improve the imaging quality of the spectral imaging system.4)This paper,the tilt and eccentricity of each element of the spectrometer system and the machining error are analyzed in terms of tolerance,and the corresponding mechanical structure is designed.While ensuring that the overall performance of the system is not affected,the size of the overall mechanical structure of the system is reduced while ensuring that each component of the system can be fine-tuned during assembly and commissioning to ensure that the system has good optical performance after the actual assembly is completed.In this paper,the cross-type Czerny-Turner structure is adopted as the optical system to meet the needs of fluorescence immunoassay analyzer in terms of spectral range,resolution,multi-channel and volume,and the aberration of the system is eliminated by the method of aspherical lens as the correction mirror,so as to improve the resolution of the system.In addition,this paper also designs a mechanical structure that meets the requirements for the use of fluorescence immunoassay analyzer,which not only meets the performance indexes of the system,but also achieves the compression of volume,and provides a multi-element analysis solution for fluorescence immunoassay analyzer.