| Objective: 1.To collect relevant literature on traditional Chinese medicine syndrome types of colorectal cancer in the past 10 years,summarize and systematically elaborate on the syndrome types of colorectal cancer;2.Collect all first diagnosed cases of colorectal cancer from the Oncology Department of Hunan Academy of Traditional Chinese Medicine in the past year,conduct statistical analysis,and study the distribution pattern between gender,clinical stage,age,site of onset,and traditional Chinese medicine syndrome types of colorectal cancer;3.To study and analyze the effects of Jianshu and Anti-Cancer Formula on the level of m6 A methylation modification during the progression of colorectal cancer,and clarify the correlation between m6 A modification and the anti-colorectal cancer efficacy of Jianshu and Anti-Cancer Formula;4.To elucidate the effects of Jianshu and Anti-Cancer Formula on the expression of methyltransferase METTL3 and METTL14,and explore the role of methyltransferase METTL3 and METTL14 in the development of colorectal cancer to elucidate the regulatory effect of Jianshu anti-cancer formula on the expression of methyltransferase METTL3 and METTL14,and to explore the role of methyltransferase METTL3 and METTL14 in the development of colorectal cancer.Methods: 1.Retrieve relevant literature on traditional Chinese medicine syndrome types of colorectal cancer published in domestic journals from January 2013 to January 2023,select literature with clear records of the specific number of cases,and conduct induction and analysis based on the number of cases,traditional Chinese medicine syndrome types,deficiency and excess in the literature to explore the laws of traditional Chinese medicine syndrome types of colorectal cancer.2.Collect all first diagnosed cases of colorectal cancer from the Cancer Department of the Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine from December 2021 to December 2022,and screen out cases that meet the inclusion and exclusion criteria.Collect the patient’s gender,clinical stage,age,location of onset,and traditional Chinese medicine syndrome type,and explore the relationship between different genders,clinical stage,age,location of onset,and traditional Chinese medicine syndrome type.3.Human colon cancer cells HCT116 were cultured for modeling,and 24 SPF-grade BALB/c nude mice were successfully modeled and divided into four groups,namely,the high-dose group of Jianpi Xiao’ai Formula,the low-dose group of Jianpi Xiao’ai Formula,the model group(saline)and the western medicine group(5-fluorouracil),and the tumors were removed and their sizes and tumor suppression rates were measured after successful modeling.4.The overall expression levels of m6 A methylation in the tumor tissues of the high-dose group,low-dose group,model group and western medicine group were quantified by colorimetric method.5.The expression levels of methyltransferase METTL3 and METTL14 in the above-mentioned different intervention groups were detected by Western blot method,and the protein expression differences between different intervention groups were analyzed.6.The expression of sox2 and SPRED2 in different intervention groups were detected using IHC method.Results: Part I: The syndrome types of colorectal cancer with a proportion of more than 10% are successively spleen qi deficiency type,damp-heat internal accumulation type,qi and blood deficiency type,and spleen and kidney yang deficiency type.Other syndrome types are less than 10%,which are relatively rare.Colorectal cancer is mainly characterized by deficiency syndrome,followed by excess syndrome,with fewer syndrome combinations of deficiency and excess.Part II: According to the etiology,pathogenesis,and symptoms of colon cancer,our oncology department divides it into 7 types: Qi and blood deficiency,incomplete stasis and toxin syndrome,Qi and Yin deficiency,heat and toxin accumulation syndrome,kidney qi deficiency,spleen and kidney deficiency syndrome,spleen deficiency and blood deficiency,stasis and toxin accumulation syndrome,dampness and heat accumulation,stasis and toxin accumulation syndrome,spleen and stomach deficiency and cold,stasis and toxin accumulation syndrome,and spleen deficiency and depression,stasis and toxin accumulation syndrome.Male colon cancer patients are mainly characterized by qi and blood deficiency and incomplete stasis and toxin,while female colon cancer patients are mainly characterized by spleen deficiency and blood deficiency,as well as internal stasis and toxin accumulation;Patients with clinical stage IV are mainly characterized by spleen deficiency and blood deficiency,stasis and toxin accumulation.Patients with stage III are mainly characterized by qi yin deficiency and heat toxin accumulation.Patients with stage II are mainly characterized by dampness heat accumulation and stasis and toxin accumulation.Patients with stage I are mainly characterized by qi blood deficiency and incomplete stasis and toxin accumulation;Between the ages of 18 and 45,the main symptoms are Qi and blood deficiency and incomplete stasis and toxin.Between the ages of 45 and 60,the main symptoms are dampness heat accumulation and stasis and toxin accumulation.Patients over the age of 60 are mainly characterized by spleen deficiency and blood deficiency,as well as stasis and toxin accumulation;Colon cancer patients are mainly characterized by qi yin deficiency and heat toxin accumulation,while rectal cancer patients are mainly characterized by spleen deficiency and blood deficiency,as well as internal stasis and toxin accumulation.There are significant differences between different genders,clinical stages,age,location of onset,and various traditional Chinese medicine syndromes of colon cancer.Part III: 1.The tumor volume in the high-dose group of Jianpi Xiao’ao Fang was smaller than that in the model group,low-dose group,and 5-Fu group(P<0.01),and the tumor weight in the high-dose group of Jianpi Xiao’ai Fang was smaller than that in the model group,low-dose group(P<0.01).The tumor inhibition rate in the high-dose group of Jianpi Xiao’ai Fang was 90.01%.2.Compared with the model group,the low dose group,and the 5-Fu group,the m6 A methylation level in the high dose group of Jianpi Xiao’ai Formula decreased(P<0.01).3.Compared with the model group,the low dose group,and the 5-Fu group,the protein expression of methyltransferase METTL3 in the high dose group of Jianpi Xiao’ai Fang was significantly lower,with a statistically significant difference(P<0.01,P<0.05);Compared with the model group,the high dose group of Jianpi Xiao’ai Formula significantly upregulated the protein expression level of methyltransferase METTL14(P<0.05).4.Compared with the model group,the low dose group,and the 5-Fu group,the expression of SPRED2 in the high dose group of Jianpi Xiao’ai Formula significantly increased,with a statistically significant difference(P<0.01,P<0.05);Compared with the model group,the expression of SOX2 in the high dose group,the low dose group,and the 5-Fu group of Jianpi Xiao’ai Formula significantly decreased,with a statistically significant difference(P<0.01).Compared with the low dose group and the 5-Fu group,the expression of SOX2 in the high dose group decreased,with no statistically significant difference(P>0.05).Conclusion: The most common type of colorectal cancer is spleen qi deficiency,followed by dampness heat internal accumulation,and the overall deficiency syndrome is the main.Therefore,treatment should mainly focus on strengthening the spleen and supplementing qi.There are also differences in traditional Chinese medicine syndrome types due to differences in gender,clinical stage,age and location of onset of colorectal cancer patients.Jianpi Xiaocancer Formula can effectively inhibit the occurrence and development of colorectal cancer,improve the tumor inhibition rate,and reduce the overall expression of m6 A methylation in human colorectal cancer HCT116 cells.By regulating the expression of METTL3 and METTL14,it can inhibit the progression and metastasis of colorectal cancer. |
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