Study Of Annexin B1 Function And Its Application To Protein Separation And Purification

Neurocysticercosis (NCC), caused by the larval stage of Taenia solium, is an important parasitic disease of the human nervous system and constitutes a public health problem. Cysticerci locats in a cavity lined by host epitheliod cell originating from small vessels, this epitheliod cell layer, which was named bladder wall (BW), constitutes the host-parasite interface in cysticercosis. Cysticerci may persist in BW for a long period of time, often for years without eliciting surrounding host inflammatory reaction. Living cysticerci may cause an asymptomatic infection through active evasion and suppression of immunity and inflammation.Annexin Bl was isolated from a library of Cysticercus cellulosae and is a novel member of annexins family and designated as annexin Bl. The annexins are a multigene family of proteins characterized by their capacity of reversibly binding to anionic phospholipids in a Ca²⁺-dependent manner. The exact role in vivo of the annexins is still unclear. As the first annexin was found in platyheminth, studies on annexin Bl can make clear what the role it plays in the infection, loading and other activies of cysticercus cellulosae, which may provide some new ways to prevent and control this diasease. Additionly, annexin Bl can also act as a molecule model for studying the evolutionary divergence of annexins.Non-fusion espression vector of annexin Bl was constrcted, the protein was expressed as soluble form in E.coli. A new purification method for annexin Bl has been developed and established. The method makes good use of the calcium-dependent phospholipids binding character which is one of the typical features of annexin superfamily. The Ca²⁺-triggered precipitation method is fast, easy to manipulate, with only a few centrifugation steps followed by resolubilization of purified protein.BALB/c mouse was immunized with annexin Bl by intrasplenic primary...