| Chemiluminescence (CL) is one of the luminescent phenomena and it can be defined as the light emission (ultraviolet, visible or infra-red radiation) produced by a chemical reaction. Although the first chemiluminescent phenomenon was reported in 1887, development of this research was slow before the latter of the 20th century. Application of the Chemiluminescence method in analysis became possible with appearance of the highly sensitive photomultipliers (PMT) in the 1950s, and now Chemiluminescence became one of the most sensitive detection methods. Due to the simplicity, low detection limit, large calibration ranges and rapid analysis speed, Chemiluminescence has been widely used in practical applications during the last 50 years. The most visible fact in the rapid development is reflected in the great numbers of publications devoted entirely to the discussion of Chemiluminescence.Current research in Chemiluminescence is focussed to two general directions. One direction is the discovery of new Chemiluminescence reactions and investigation of their applicability to real samples and the other direction is the development of Chemiluminescence detection systems for newly developed separation techniques.In this thesis, the principle of Chemiluminescence, bioluminescence and electrochemiluminescence, and research works in recent years about Chemiluminescence coupled with capillary electrophoresis and liquid chromatography, Chemiluminescence sensor and application of Chemiluminescence in biochip were reviewed. The present research work was focussed on the new Chemiluminescence systems, novel flow-through Chemiluminescence biosensors. The major content was described as follows:1. Chemiluminescence analysis with on-line electrogenerated reagents as the oxidantSince Abbort et al reported the CL determination of morphine and its derivation by oxidation with potassium permanganate, there have been increasing interest in theapplication of inorganic oxidate-based CL reactions to analytical chemistry. Inorganic oxidants, such as H202, Br2, KMnO4, Ce(IV), [Ru(bpy)3]2; [Fe(CN)6]3+, 10;, CIO', BrO" and BrO3", were frequently used in CL reaction; however, this method can not be employed if the reagents are not stable for at least the duration of the experiment. For example, the disproportionation of CIO' and BrO" is very easy in solution, so their applications in CL analysis are limited.The application of strongly oxidizing reagents in quantitative analysis has in general been restricted to those reagents that are not or only very slowly reduced by water, thus making possible the storage of standard solutions. Stronger oxidants, such as cobalt(III), silver(II) and manganese(III), are quickly reduced by water, so their application in quantitative analysis under normal conditions is not possible.The success of flow injection analysis (FIA) can be attributed to the reproducibility of the operations involved in the system, such as injection, pumping and timing, allowing quantitative analysis without the necessity of completeness of the chemical reaction. As a complete chemical reaction is unnecessary, the idea arises that instability of the reagent also might be no objection as long as the reaction rate is reproducible. This would open possibilities for the application in quantitative analysis of these reagents, which are unstable under normal condition, by generating them in flow systems, starting from a stable oxidation state of the same element. In flow system, the unstable solution generated by chemical oxidation contains some superfluous oxidant, which interfere with the subsequent reaction. The electrochemical generation does not have the drawback mentioned above.In this thesis, the investigating aim of the research work was focus on the development of new oxidants in CL analysis to extend the analytical application fields or to improve the analytical performance of some CL analysis relating to unstable oxidic reagents by combining flow-injection technique. Some reagents needed for the CL reaction are ele...
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