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Study And Application On Methods And Mechanism Of Separation Of Crocin In Saffron (Crocus Sativus L.)

Posted on:2002-02-07Degree:DoctorType:Dissertation
Country:ChinaCandidate:H ZhangFull Text:PDF
GTID:1101360092475716Subject:Leather Chemistry and Engineering
Abstract/Summary:PDF Full Text Request
A preparation method using low-pressure liquid chromatography to isolate crocins, the most abundant compounds in the saffron hydroalcoholic extract, has been developed. A material, which banded n-butane styrene-divinylbenzene polymer, has been employed as the stationary phase. The best separation is obtained by gradient elution using methanol aqueous solution as mobile phase. Yellow pigments of saffron have been separated to twenty fractions. Among them, the purity of ten compounds is as high as 90% by using a purification column respectively. The purity of crocin-1 is 98.4%. Because crocin-1 is the most abundant in yellow pigments of saffron, such high purity crocin-1 can serve as the standard sample of medical saffron. The structure of crocin-1, crocin-2 and crocin-3 has been detected by UV-Uis, IR, MS, 'HNMR and 13CNMR. The structure of other compounds has been conjectured by UV-Uis, MS.SZ-1 adsorbent resin has not been large surface areas and excellent porous structure, but also have high mechanical strength and thermal endurance. Moreover, the adsorbent resin also has high oxidation endurance and bio-degradation endurance, as well as acidic and basic degradation. The comprehensive studies oh the adsorption properties of SZ-1 adsorbent resin were conducted in way of mobile adsorption and various effect factors were also studied. The separation mechanism was explored.A simple sensitive and specific high-performance liquid chromatography -UV (HPLC-UV) has been developed for quantify the major biologically active ingredient of saffron, namely crocin-1. The HPLC assay was performed on a reversed -phase C18 column using acetonitrile -tetrahy-1% aqueous acetic acid as mobile phase. Calibration was linear (R = 0.9996), with intra-and inter-day RSD of less than 9%. The assay was successfully applied to determination of four saffron samples. Result indicates that the developed HPLC assay can be readily utilized as a quality control method for crocin-containing medicinal.A method, determinate Se in saffron using AFS-230 hydride generation atomic fluorescence spectrometry (HG-AFS), was studied. The optimal analytical conditions were examined. The detection limit was 0.5 μg/L, the linear range was 1.5 - 15.0 μg/L, the correlation coefficient was 0.9999, and the recovery rate was about 90%-97%. Se of saffron in 4 regions was determined by standard curve method. The experiment results show that the developed HF-ASS assay can be readily utilized as measure for determination Se in saffron.
Keywords/Search Tags:saffron, separation, preparation, separation mechanism, HPLC assay and Se
PDF Full Text Request
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