Studies On Properties Of Collagen Extracted From Hideoffals And Its Blend Biomaterials With Polysaccharides

Hideoffals as a major part of tannery waste contain over 80% collagen. Collagen is a kind of excellent functional biomaterial as well as a good source of amino acids. Because of its special biological properties, collagen has good application foreground in medicine, food, cosmetic and agriculture.Based on present application status of waste products in curriery in China, the advantages and disadvantages of collagen and analysis and characterization of collagen extracted from hideoffals, studies on collagen application properties and its blend films with one or two polysaccharides were done in this thesis.The contents and results of this thesis are as follows:First, relative molecular weights of collagen and its polypeptides were determined. It is difficult to determine molecular weights of collagen and its polypeptides with relative molecular weights less than 20 kD. Aimed at this, a new method to determine relative molecular weights of small molecular polypeptides was set up. This method is especially suitable for the measurement of relative molecular weights of small molecular collagen and its polypeptides.Second, a systemic analysis on physicochemical properties of collagen and its polypeptides with different relative molecular weights shows that collagen and its polypeptides have good application properties. Determination on viscosity property of collagen and its polypeptides with different relative molecular weights indicates that the viscosity of collagen and its polypeptide solutions is not only changed withtheir relative molecular weights but also can be influenced by various factors such as concentration, temperature, acidity, electrolytes added and keep time.Third, when collagen is used in tissue engineering, it has some drawbacks such as poor mechanical property, higher water absorbability, much lower ability to be moulded at watery condition which is unsuitable for construction of inner organs, much low stability to collagenase produced in the body and its cambium and low ability to synchronize with the growth rate of tissue cells. Based on the above, three novel kinds of collagen-polysaccharide blend films are first made including collagen-Konjac glucomannan blend film (C-KGM), collagen-Konjac glucomannan-chitosan blend film (CKCS) and collagen-Konjac glucomannan chondroitin sulfate blend film film (CKCs). FT infra red analysis (FT-IR) and X-ray diffraction (X-RD) were applied to study morphological structure, mechanical property, water absorption and heat stability of the blend films and the interactions and compatibility of the blend. X-ray photoelectron spectrum (XPS) and determination of surface energy were also used .to study the surface property of CKCS and CKCs. Biodegradability of Konjac glucomannan gel and CKCS in vitro was tested by mimicking physiological conditions in vivo. Biocompatibility of CKCS and CKCs. was determined by cell culture in vitro, dynamic blood coagulation and hemolysis test. In addition, animal experiment was applied to investigate some of medicinal properties of these two blend films.Fourth, results from studies on structure characterization and biological properties results show that collagen can be miscible with Konjac glucomannan at any ratio at pH value of or lower than 7.0 with stronger interactions and good compatibility being present between collagen and Konjac glucomannan in the blend film at this condition. The freeze-dried collagen-Konjac glucomannan film cross linked by glutaraldehyde has a porous structure essential for hemostasis materials. Compared to pure collagen film, the film has higher stability to heat and bettermechanical property. However, this blend film has much stronger water absorbability. Animal experiment shows that cross linked freeze-dried film has better hemostasis and adhesiveness than gelatin sponge, and that a cross linked freeze-dried film prepared by collagen and Konjac glucomannan in a certain ratio can produce hemosatsis close to that of pure collagen sponge: As a good hemostasis material, this film will be...