Preparation Of Polysaccharide Derivative Chiral Stationary Phases And Their Application In High-Performance Liquid Chromatography And Capillary Electrochromatography

Preparation and application of covalently bonded polysaccharide derivative chiral stationary phases (CSPs) is one of the promising research fields in separation sciences. The objective of this thesis is to develop new methods to prepare covalently bonded polysaccharide derivative CSPs for separation of enantiomers by high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC).An improved diisocyanate method was proposed to prepare covalently bonded cellulose trisphenylcarbamate derivative CSPs by changing the synthetical procedures. By using the improved method, the cross-linking of the glucose unit occurred in the classical diisocyanate method was avoided. Cellulose derivatives could be attached by regio-nonselective approach, while minor loss in the chiral resolving ability was observed.A radical co-polymerization method was developed to immobilize polysaccharide derivatives onto vinylized silica by regio-nonselective or selective approaches. CSPs prepared with regio-nonselective approach showed similar chiral resolving abilities as those prepared with regio-selective one, however, the former approach was more promising from the view point of rapid preparation. CSPs prepared by this method possessed higher chiral resolving ability than those prepared by previously reported methods.