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Enzymatic Bioconversion Of Organsilicon Compound In Non-aqueous Phase

Posted on:1997-10-13Degree:DoctorType:Dissertation
Country:ChinaCandidate:S Y QiuFull Text:PDF
GTID:1101360185464899Subject:Fermentation engineering
Abstract/Summary:PDF Full Text Request
Enzymatic catalysis in non-aqueous phase was one of the most important field in enzyme engineering.Lipase from Candida cylindracea and immobilized lipase Lipozyme catalysed organsilicon compounds in organic medium was studied.The possibility of esterification of non-natural organsilicon compound trimethylsilyl-alcohol with fatty acid catalyzed by lipase from C. cylindracea was explored and the effect of various factors on the reaction was studied systematically.Lipase from C.cylindracea could catalyze esterification of trimethyisilyl-alcohol with fatty acid in organic solvent.The optimal amount of lipase was 50mg. Organsilicon compound was better acyl-acceptor. Enzyme activity decreased when the molecular weight of organsilicon alcohol increased.The enzyme could catalyze a different fatty acid substrate.Enzyme activity was inhibited by high concentration fatty acid but not inhibited by trimethyisilyl-alcohol. Enzyme activity increased with hydrophobicity of organic solvent increaced Enzyme had the largest activity in octane but no active in tetrahydrofuran.The optimal water contents of enzyme reaction in hexcone was 0.06(W/V)%.The optimal temperature changed when the water contents of organic solvent changed.Lipase from C.cylindracea could catalyze transesterification of trimethyisilyl-alcohol with fatty acid ester in organic medium. Enzyme activity was not inhibited by high concentration organsilicon alcohol and fatty acid ester.Conversation ratio of transesterification was higher in water saturated octane than that in water removal octane.The possibility of esterification of non-natural organsilicon compound trimethyisilyl-alcohol and fatty acid catalyzed by immobilized lipase Lipozyme was studied and the effect of various factors on the reaction was discussed systematically. Immobilized lipase Lipozyme could catalyze...
Keywords/Search Tags:Bioconversion, Non-aqueous phase, Lipase, Organsilicon compound, Esterification, Interesteriification, Ultrasound
PDF Full Text Request
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