Development And Application Of 180°Backward Light Scattering Technique

Since its simplicity and high sensitivity, resonance light scattering (RLS) technique, developed more than ten years ago, has attracted considerable attentions and become widely applicable in the determination of nucleic acids, proteins, carbohydrates, metallic ions, drugs, surfactants, nanoparticles, bacteria etc. In principle, RLS technique is mainly based on the detection of scattering signals at right angle. A few reports have displayed based on the detection of backward light scattering signals, and there are few reports at present involved in the 180° BLS signals. Based on the background, concerning the application, development and disadvantage of RLS technique, a RLS technique based on 180° BLS signals detection has been developed in this work.The main investigated contents are as follow:1. The spectral characteristics of the interactions between Biebrich Scarlet (BS) and human serum albumin (HSA), bovine serum albumin (BSA) and γ-Globulin (γ-IgG), lysozyme (Lys) have been investigated using RLS technique and then constructed a flow injection-resonance light scattering (FI-RLS) technique for the detection of proteins. The experimental results shows that the FI-RLS technique could reduce the relative standard deviation (RSD) from 2.43% to 0.79% and can be applied to the analysis of instable system. FI-RLS technique facilitates automatic and on-line analysis, and can also be adaptable to...