Application Of Novel Resonance Light Scattering Technique In The Interaction Of Organic Micromolecular Polluter With Biomacromolecule

Recently, RLS technique attracted attention due to its simple manipulation, high sensitivity and convenient experimental conditions. Moreover, RLS technique has been extensively and successfully utilized in various fields. For example, RLS technique has been applied to the characterization of supermolecular assemblies, the research of interaction of dye and biomacromolecule such as protein, nucleic acid. Nowadays, RLS technique has become a powerful and routine assay in analytical chemistry. However, with the further development of RLS technique, the limitations of RLS technique are clarified gradually. First, the maximum RLS intensities at characteristic wavelengths were not reliable indicators in practical operation to describe the properties for RLS spectra. Second, RLS technique as a routine analytical method is still not applied in on-line detection, actually affecting its promising prospect in applied science. To overcome these drawbacks, we innovatively combine flow rejection analysis (FIA) with RLS technique and successfully develop the FIA-RLS coupled technique; and we also developed dual-wavelength resonance light scattering ratiometry and plasmon resonance light scattering technique. As fellows:The first part of this contribution is to develop the FIA-RLS coupled technique and its application in the interaction of dye and heparin, the following is the abstract:In near neutral medium, the binding of heparin with methylene blue (MB) occurs and results in enhanced resonance light scattering (RLS) signals characterized at 365.0 nm. It was found that the enhanced RLS intensity (â–³I_rls) is proportional to the concentration of heparin, and thus a flow...