Preparation And Application Of Novel Polymer Monolithic Capillary Columns In Pressurized Capillary Electrochromatography

Capillary electrochromatography (CEC) has been considered as a very promising analytical technique that combines the efficiency of capillary zone electrophoresis and the selectivity of liquid chromatography with the use of stationary phases.As we know, the analysis of strongly basic analytes is still an analytical challenge for many years because traditional bonded stationary phases exhibit a high density of silanol groups, which results in secondary interactions, severe peak tailing and increased retention. In second chapter, a monolithic stationary phase was prepared in a single step by the in situ copolymerization of iso-butyl methacrylate, ethylene dimethacrylate and N, N-dimethylallylamine in the presence of porogens. The amino groups on the stationary phases are meant to support the electroosmotic flow (EOF) from cathode to anode necessary for moving the mobile phase through the monolithic capillary. Basic compounds such as aniline was well separated on the monolithic columns in the'counter-directional mode'to avoid the electrostatic adsorption between basic analytes and the charged groups on the surface of the stationary phases. In third chapter, monolithic columns with in situ polymerization of iso-butyl methacrylate, ethylene dimethacrylate and methacrylic acid were prepared. Peak tailing of basic compounds such as anilines was likely to avoid by using ion-suppressed mode of CEC.The possibility of rapid separation resulted from the unique pore structure with high permeability and favorable mass transfer characteristics of the monolithic stationary phase. In fourth chapter, five anthraquinones including emodin,...