On The Mixed System Of Plant-bacteria For Bioremediation Of The Azo-dye Wastewater

The azo-dye wastewater is characterized with high value of COD_cr, complication of organic components, more difficult remediation, high colority, biologic poison and so on. At present, the economic and efficient treatment of this wastewater is still a difficult problem, and research on the treatment biotechnology is a very important signification.In this paper, according to the character of the azo-dye wastewater and bacteric biotechnology, photoremediation and physical adsorption technology, the effective system of the mixed bacteria-rice-natural zeolite was founded. The dye of Amido Black 10B was biodegraded in this system.Firstly, the cultivation of the mixed bacteria, biodegradation of Amido Black 10B, the kinetic equation of biodegradation and the production of Amido Black 10B biodegradation were researched in detail. Furthermore, it was also studied that the natural zeolite and the modified natural zeolite adsorbed the dye Amido Black 10B under a different condition. Finally, with the potted plant, the Amido Black 10B was biodegraded with the system of the mixed bacteria-rice-natural zeolite in greenhouse. It was researched that their interactions among the mixed bacteria-rice-natural zeolite, the effect on the development of rice by dye, efficiency of the system for biodegradation of Amido Black 10B and so on. With these experiments, the following results can be obtained,(1) The mixed bacteria contain 70% of microzyme and lactobacillus, and the other is photosynthetic bacteria, actinomyceten, mildew bacteria and so on. The kinetic equation of the mixed bacteria bioremedating of the dye of Amido Black 10B is fit to the stair kinetic equation. The process of bioremediation is formation of a small molecule aromic ammonia through decomposing the construct of——N(?)N——(2) The acidity, the dosage of natural zeolite, adsorption time, the content of Amido Black 10B and so on, had an important effect on the adsorption of dye Amido Black 10B by the natural zeolite. The dynamic equation of adsorption is fit to the Langmuir isotherm and Freundlich isotherm.The adsorption rate of Amido Black 10B into the modified natural zeolite is higher than the natural zeolite. The multi-technologies modified natural zeolite is best in the adsorption of Amido Black 10B, and the rate of adsorption is 60.04% in the light.(3) The dye of Amido Black 10B had an important effect on the development of the rice, and could modulate the interaction between the mixed bacteria and the rice. That is, along with the content of the dye of Amido Black 10B increasing, the development of the rice was restrained seriously. The flow of interaction between the mixed bacteria and the rice appeared from the negative interaction to the positive interaction, and appeared zero at last.(4) The efficiency of the system of the mixed bacteria- rice - the natural zeolite is higher than the system of the mixed bacteria-the natural zeolite, or the system of the natural zeolite-rice. The biodegradation rate of Amido Black 10B(concentration is 40mg/kg) is 69.25% in the system of the mixed bacteria- rice - the natural zeolite.(5) The model of biologic chemistry and molecular dynamic mechanism for biodegradation of Amido Black 1 OB in the system of the mixed bacteria-rice-natural zeolite could be simulated by this equation, the results from this equation is good fit to the results obtained from this study.The results provided basis for the application of the system of the natural zeolite-rice-the mixed bacteria to the bioremediation of azo-dye wastewater. The successful application of the system of the mixed bacteria -rice-the natural zeolite will provide reference for the eco-treatment of the other type of wastewater.