Screening And Molecular Biological Identification Of Phenol-Degrading Halophilic Microorganisms

The study on the treatment problem of phenol wastewater under high salinity condition is the present focus on environmental science and engineering fields in China.Isolation and screening of halophilic microbial strains , determination of their taxonomic status using molecular biological techniques,phenol-degrading efficiency of these microbial strains, combined microbial consortium and compared of the treatment performance using different treatment methods were made.They offered the microbial strains,data support ,experiment techniques and scientific guidance for the treatment of high salinity phenol wastewater in industry.Systematically summarized the development of research at home and abroad on high-salinity wastewater containing phenol,this paper focused on the following four research works:1. Screening and molecular biological identification of phenol-degrading halophilies from salt pondsHalophilies were isolated from salt ponds of Lianyungang Tai Nai District,and two halophilic microbial strains designated HBCC-2 and HBCC-3 were screened respectively. HBCC-2 and HBCC-3 were identified according to the biochemical and physical characteristics ,16S rRNA gene of the two microbial strains were sequenced and analyzed. They could use phenol as sole carbon source afer being acclimated for about three years by phenol ,and the efficiency of phenol treatment of HBCC-3 was better than HBCC-2.2. Screening and molecular biological identification of phenol-degrading halophilies from activated sludgeFour middle halophilic microbial strains were isolated from activated sludge of a operating coking plant, respectively.They were designated as JHCFS1, JHCFS2, JHCFS3 and JHCFS4 respectively. JHCFS1, JHCFS2, JHCFS3 and JHCFS4 were identified according to the biochemical and physical characteristics and 16S rRNA gene of the four microbial strains were sequenced and analyzed. The efficiency of phenol treatment were tested by them when NaCl and KCl concentration were 30g/L respectively. The result illustrated the best treatment performance of wastewater was used of JHCFS2 and the worse treatment performance of wastewater was used of JHCFS4. 3. Screening and molecular biological identification of phenol-degrading halophilies from soil polluted by coking wastewaterOne halophilic and phenol-degrading microbial strain designated as CC2 was isolated from polluted soil of a abandoned coking plant.16S rRNA gene of CC2 was sequenced and analyzed, it was identified according to its biochemical and physical characteristics.The phenol hydroxylase gene of CC2 was detected directly using the pair of primers Ph1 and Ph2.The phenol degradation characteristics of CC2 was tested,the resulted showed CC2 could use phenol as a sole carbon source when NaCl and KCl concentration were 30g/L for its growth, respectively,4. Research on high salinity phenol wastewater treatment performance by halophilic consortiumThe treatment performance of high-salinity phenol wastewater was investigated and compared by halophilic microbial consortium consisting of different mixture ratio of seven phenol-degrading halophilies at a orbital shaker. Based on the best treatment efficiency of phenol by combined microbial consortium , the treatment performance by four different methods, including suspension of microorganisms,absorption of activated carbon,embedding immobilization by sodium alginate, embedding immobilization by sodium alginate and active carbon simulating SBR reactor equipment were investigated repectively,the result showed embedding immobilization by sodium alginate and active carbon was best one among the four methods.