Screening Of Phenol Degradation Of Bacterium And Its Characteristics

With the development of economy and industrialization, the environment is threatened by phenol wastewater which need be dealed with as soon as possible. Compared with physical and chemical methods, using method of microorganism biodegradation to treat with phenol wastewater is with lower cost, no secondary pollution and effective.One phenol degrading microorganism named JFG0601 was isolated acclimating was carried out by way of gradually increasing dosage of phenol (as the sole source of carbon) in constant time. The phenol-degradation tests proved that JFG0601 had the strongest phenol-degradation capacity, which could degrade 1800mg·L^-1 phenol and the degradation of phenol is up to 85.49% within 48 hours. The optimum environmental conditions for phenol degradation is 30～40℃,pH 6～9,150r·min^-1,the phenol solution(50mL) was added into triangular flask(250mL).One phenol degrading microorganism named JFG1201 was isolated using ultraviolet mutation method. The phenol-degradation tests proved that JFG1201 had the strongest phenol-degradation capacity, which could degrade 2200mg·L^-1 phenol and the degradation of phenol is up to 81.59% within 48 hours. The orthogonal tests prove that the strain of JFG1201 can growth good: 30℃,NaCl 0.2g·L^-1,pH 7 and NH₄NO₃ 0.8g·L^-1.According to the microscope observation, physiology and biochemistry experiments of the strain of JFG1201 and on the basis of identification and categorization of bacteria, we can identify preliminarily it as Pseudomonas sp.By PCR and electrophoresis analysis, the complete sequence of the JFG1201 strain's 16S ribosomal DNA was found to be homogeneous over the rate of 99% to many members of Pseudomonas sp. in GenBank of the Internet, so we can identify it as Pseudomonas sp. at molecular level.Moreover, PCR and electrophoresis analysis detected a typical band of DNA fragment of phenol hydroxylase. The nucleotide sequence of this fragment and its deduced amino acid sequence share 99.3% and 99.3% homology with the phenol hydroxylase gene and its deduced amino acid sequence of phenol-degrading strain Pseudomonas sp.D28864.1, which can confirm that the function of phenol-degrading capability was examined at molecular level.