Scanning Probe Microscopy Imaging Of Biological Origin And FDTD Simulation Of Optical Tweezers

This thesis describes the biological applications of scanning probe microscopy (SPM) and the study of the optical trapping theory. For the former, biological imaging study of atomic force microscopy (AFM), atomic force/photon scanning tunneling microscope (AF/PSTM) operating, and biological imaging of AF/PSTM are elucidated. For the later, application for the optical tweezers theory using the finite difference time domain (FDTD) method is discussed.Scanning probe microscopy is one of powerful techniques studying the solid body surface with high spatial resolution. AFM, overcoming the limitations in imaging non-conducting samples, and allowing measurements of native biological samples in physiological-like conditions, becomes an important tool to the study of samples of biological origin. This thesis illustrates the clinical and environmental researches of atomic force microscopy, utilizing AFM imaging of proteus, canine renal epithelial cell and influenza viruses. In the AFM images of canine renal epithelial cell, the granules and the hole on the cell surface are shown, and the structure of glycocalyx on the cell membrane is obtained. In the AFM images of influenza viruses, the glycoprotein protrusions on the virus surface are shown.Atomic force/photon scanning tunneling microscope which combines AFM and photon scanning tunneling microscope, can implement the AFM imaging, and eliminate the spurious image with two-beam symmetric illumination system. With the method of separating image, the transmittivity and refractive index images of sample are obtained using AF/PSTM. In this thesis, the operating of AF/PSTM is discussed, that is how to perform the functions of AFM and PSTM imaging simultaneously. The function of AF/PSTM is validated by imaging research of a variety of samples. Biological applications using AF/PSTM are studied, and it is shown that AF/PSTM is one of powerful techniques in the study of life science. Especially, in the AF/PSTM images of the spermary of reproduction system of Argotecten irrabians, the ultramicroscopic structures of the sperm karyon is shown through transmissivity and refractive index images, while the its topography is obtained.Optical tweezers is a term describing the manipulation of microscopic and mesoscopic particles using the light radiation pressure force. Non invasive manipulation of biological...