| In this paper, several diagnostic tests for chicken infectious anemia (CIA), including enzyme-linked immuno-adsorbent assay (ELISA), indirect fluorescent antibody (IFA), and polymerase chain reaction (PCR) were developed and comparatively studied. Superoxide Dismutase(SOD), Glutathione peroxidase(GSH-P,? Malonaldehyde diethyl acetal(MDA), and trace elements in the sera from 1-day-old chickens infected with chicken anemia virus (CAV) were also detected. Moreover, to shed a light on the pathogenesis and immunological mechanism of SPF chickens experimentally infected with CAy, we assayed the kinetics of CD3~, CD4~, CD8~, and I-lymphocyte subpopulations and B-lymphocytes in the thymus, spleen, and bursa of Fabricius of CA V-infected 4-week-old chickens. The results are show as.1.ELISA, IFA, and PCR assays were developed and used to detect the infected 1-dayold chickens 7, 14, 21, and 28 days post-infection (p.i.). The results showed that the detection frequencies were 0/10, 5/10, 8/10, and 10/10, respectively, for ELISA, 0/10, 3/10, 5/10, and 10/10 for IFA, and 10/10, 10/10, 10/10, and 10/10 for PCR. This indicated that PCR was more sensitive than the other two during early infection, which were equally applicable for the diagnosis of CIA 4 weeks p.i.2.SOD, GSH-P~, and MDA in the sera from 1-day-old SPF chickens infected with CAV were detected 7, 14, 21, and 28 days p.i.. A dramatic decrease of SOD and GSH-P~ and a significant increase of MDA were found in sera of infected chickens compared withthat of uninfected control chickens. This showed that the ani-oxidizaion function wasimpaired and the ability of clearance of free radical was reduced in the infected chickens.3. The trace elements associated with anti-oxidant fiJnction were detected in the bloodsamples from CAV-infected l-day-old chickens. Se, Cu, Zn, Mn, and Fe were significantlydecreased. It was implied that altCration of inner environment occurred in the infectedchickens, and that the falls in Se, Zn, and Mn contentS positively correlated with the SODactivity.4. The CD3+, CD4+, CD8+, and T-lymphocyte subpopulations in the peripheral blood ofCAV-infected 4-week-old chickens were assayed by flow cytometry (FCM) 7 to 21 days p.i..Declines of the four subpopulations of T-lymphocytes were found in infected chickenscompared with uninfected controls, with significant difference 7 and 2l days p.i. (P<0.05),extremely significant difference l4 days p.i. (P<0.0l), and no statistical difference 28 daysp.i. and thereafter (P>0.05) for CD3+ T-lymphocytes; with eXtremely significan difference 7to l4 days p.i. (P<0.0l), significant difference 2l days p.i. (P<0.05), and no statisticaldifference 28 days p.i. and thereafter (P>0.05) for CD4+; with significan difference 7 and28 days p.i. (P<0.05), and extremely significan difference l4 to 2l days p.i. (P<0.0l) forCD8+; with significant difference l4 days p.i. (P<0.05), extremeIy significant difference 21days p.i. (P<0.0l), and no statisticaI difference during other time for. These foundingsindicated that CAV infection had an adverse impact on T-lymphocytes of mostsubpopulations.5. The CD3+, CD4+, CD8+, and T-lymphocyte subpopuIations in the thymus of CAV-infected 4-week-old chickens decreased dramatically. CD3+ T-lymphocytes reduced by 50%or so, CD4+ and CD8+ were impaired greatly, almost dying up in the cortex and onlyreticulum left. And T-lyInphocytes also dropped aPparently. These showed that CAVinfection mainly damaged immature T-lymphocytes, which is consistent with the previousfOundings.6. The CD3+, CD4+, CD8+, and T-lymphocyte subpopulations in the spleen of CAV-infected 4-week-old chickens also decreased dramatically. CD3+ T-1ymphocytes dropped by50% or so 7 days p.i., the other populations were significantly less than that of the controls,especially l4 days p.i.. These indicated that CAV impaired not only the T-lymphocytes incentral immune organs, but also those in peripheral immune organs.7. By immnohistologica... |
PDF Full Text Request