Expression Of Bovine Ephemeral Fever Virus Transmembrane Glycoprotein Gin Recombinant Vaccinia Virus And Baculovirus

Bovine ephemeral fever virus (BEFV) is an arthropod-borne rhabdovirus, which causes a disabling febrile infection of cattle and water buffalo. The disease is common in tropical and subtropical regions of Africa, Asia, Australia and the Middle-east and is of major economic importance to dairy and grazing industries. The surface glycoprotein G is the major neutralizing and protective antigen of BEFV. In this report we cloned the gene of 6 protein from the genome RNA of BEFV Chinese isolate JB76H by RT-PCR. The nucleotide sequence of this gene showed 91% homology compared with Australia isolate BB7721.This indicated that the difference of area might cause the change in the G sequence. In order to kxow whether the G gene can be expressed in recombinant vaccinia virus (rVV) and baculovirus (rBV), we constructed BEFV 1B76H G recombinant vaccines virus and bascules virus. The results showed that the G gene couldn be expressed optimally in rVV under the promoter P7.5 for the existence of T5NT sequence in the G gene. However, the G gene could be expressed optimally by using the synthetic vaccinia P promoter that was very strong in rVV. The result showed that the G protein expressed by rVVPG could induce significant high neutralizing antibodies to BEFV in rabbit. This showed that rVVPG had the potential ability to be a recombinant vaccine for BEFV. We also identified that the G protein expressed by rBV could induce neutralizing antibodies to BEFV in rabbit and react with BEFV antiserum from cattle. Studying on the distribution of lymphocytes subpopulation in the peripheral bovine blood was conducted. The result showed that the C04+ cell could be increased alter immunized by the vaccina, BEFV or inactivated BEFV virus.