The Systematic Analysis And Toxic Comparative Pathology Research Of Locowed (Oxytropis Kansuensis) Alkaloid Omposition

The experiment has achieved the follwing results through the systematic analysis and toxic comparative pathology research of locoweed (0. kansuensis) alkaloid composition.1.The extraction of alkaloid from Oxytropis kansuensisThe systematic extraction result of alkaloid of 0. kansuensis indicates that a Kansuensis contains alkaloids of minimum, neutral and maximum polarity,of which maximum polarity alkaloid hold the firstplace.With TLC checking on alkaloid from different polarity phase, it doesn' t contain quinolizidine alkaloids-anagyrine, thermopsine etc, but indolizidine alkaloids-swainsonine and so on. Swainsonine is the main composition in maximum polarity alkaloids.2.The fractionation of alkaloid from O.kansuensis by column chromatographyWe applied silica gel column chromatography to get the fractionation of alkaloid from a kansuensis and get 2 monomer compound (crystal A and crystal D) and alkaloid mixture of B,C,E,F phase. Crystal A is a light yellow needle with purple fluorescence and test with ameliorated dragendorff reagent shows faint colour. Crystal D is a yellow plate with purple and dark fluorescence, tests with sulpharic alcohol and ameliorated dragendorff reagent show yellow and brown respectively.The test withameliorated dragendorff reagent in phase B shows 5 orange spots. Test with Ehrlich's reagent shows 5 to 8 red purple spots in C,E and F phase.3.The identification of alkaloid from O.kansuensis41 kinds of alkaloid in the maximum polarity from 0. kansuensis were identified by GC-MS. The maximum polarity of alkaloid were treated by acetylation and zinc reduction and 5 kinds of alkaloid composition were identified by HI-MS. they are swainsonine, swainsonine N-oxide, lentiginosine,retroecine and 2-hydroxyindozidine,the last two are discovered for the first time from the plant.Crystal A is initially identified as N-benzoylglycine methyl ester through IR, MS, 1HNMR crystal D is identified as 5, 7-dihydroxy-4' -methoxyflavone through UV, IR, 11-INMR, '3CNMR, the compound was discovered for the first time from the plant.4.Related analysis of alkaloids from A. variabilis,Q. Iatiracteata and0.Kansuensis by thin layer chromatographyA related analysis of alkaloids from A. variabilis, 0. latiracteata and 0. Kansuensis by means of TLC indicated that the types of alkaloids of these three locoweeds are alike,as they all belong to indolizidine alkaloid. From the quantity in different phase of polarity, it can find that the alkalods of these three locoweeds mainly localized in n-butanol segment with swainsonine as its main composition.5.The study on toxicity of alkaloid from O.kansuensisPour mouse and sixteen chickes were dreched with 800mg/kg of alkaloid from 0. kansuensis per day. In 15th to 20th day, the typical pathological change from locoweed poisoning can be duplicated. The liver, kidney and other solid organs cytoplasmic vacuolation indicated that the main toxicity composition is swainsonine.6.The extraction, isolation and identification of alkaloids formThe rmops is Ianceo lateThe extraction, isolation and identification of alkaloids from Thei-mopsis lanceolate indicate that dried Thermopsis lanceolate contains 1. 90% of alkaloid. 12 types of quinolizidine alkaloids were identified through the GC-MS from total alkaloid, of which 5 kinds of compound were discovered for the first time in this plant.7.The study on toxicity of Thermopsis IanceolateThe research on the pathology of poisoning goat indicated that the pathologic histology changes are mainly granular degenration in viscera cell in particular and hyperplasia of connective tissue and bile duct of liver.The ultrastructural change of liver shows collagenous fiber hyperplasia as its feature which is the typical change of cirrhosis of the lives. These pathological changes are totally different from locoweed poisoning.