Oligosaccharides Function And Effect On Weaning Piglet Performance As Well As Non-Specific Immunity

The effect of three oligosaccharides on weaning piglet's performance and non-specific immunity were examined in mis trial. Duroc×Landrace × Harbin white piglets from 15 litters at similar age were randomly grouped to 5 and another 1 litter as additional treatment, that was treated as control, fructo-oligosaccharide (FOS), iso-malto-oligosaccharide (IMO), manno-oligosaccharide-Protein (MOS-Pr), probiotics (Probio) and additional tylosin (Antibio). Oligosaccharides were offered in feed between 7th days before weaning and 14 days after at 1.0g/kg, probiotics (Lactobacillus acidophilus, Streptococcus faeciian, Baccillus cereus, Bifidobacterium Infantis) at 3×1014/daypiglet and tylosin at 100 mg/kg. At the end of the period, 3 average bodyweight piglets from each litter were electric paralysed for collecting blood and serum, digesta, and ingtestine wall samples for analysis. Liver, spleen and intestine physical morphology characters were measured. The effect of fructo-oligosaccharide on E.coli electricity was tested by thin layer solution electrophoresis. The bacteria adhesion on separated ileum mucosa cell that treated with oligosaccharides was examined. Separated intestine part was injected with fructo-oligosaccharide and analyzed with electro-microscope. The E.coli un-fermentable component of oligosaccharides was tested.Comparing with control, the bodyweight gain was increased (+)/decreased (-) by +32.63% in Probio (p=0.001, SPSS), +23.12% in MOS-Pr (p=0.022), +14.96% in FOS (p=0.136), +5.78% in IMO(p=0.571) and -11.82% in Antibio(p=0.093). There was 7.0% improvement on feed efficiency and the lowest diarrhea rate in MOS-Pr. Diarrhea rate was reduced in FOS and in Probio in the beginning 4 days of weaning. Liver weight was apparently decreased by 7.9% and spleen weight decresed by 11.2% in MOS-Pr. Spleen weight was apparent decrease by 23.4% in Probio and by 27.3% in Antibio. Small intestine length was decreased by 15.5% in MOS-Pr and decreased by 21.4% in Probio. Small intestine weight was decreased by 21.9% in MOS-Pr and by 39.8% in Probio. Small intestine length was increased by 10.2% and small intestine weight significantly was decreased by 51.2% in Antibio. Caecum length was significantly increased by 36.7% in Antibio and decreased by 11.1% in Probio.Comparing with control, caecum digesta acidity was unsignificantly decreased by 10.9% in MOS-Pr and increased by 12.4%~19.2% in other treatments. Caecum digesta NH4* activity was decerased by 25.2%~42.9% in tested treatments; Near end ileum digesta NH4* activity was decreased by 13.2%~47.7% in tested treatments except increased by 24.4% in Antibio. Nosignificant difference on serum NH4+ activity was found between treatments. Comparing with control, digesta nitrite activity/nitrate activity (Ni/Na) value varied unparallel between serum, caecum and ileum and changed irregularly; Ni/Na value showed parallel between serum and caecum digesta in MOS-Pr; and Ni/Na was lowed in Probio; Ni/Na value was unsignificantly increased in caecum and decreased in near end ileum. Near end ileum digesta lactobacillus acidophilus counts lied in 107.5/ml~lo9/ml and E.coli counts lied in 106.3/ml~10Vml, caecum digesta lactobacillus acidophilus counts lied in 108.3/ml~109.3/ml and E.coli counts lie in 10Vml~107 Vml, no apparent differences between treatments were found. Supposing the balabnce of Lactobacillus acidophilus I E.coli was 1.00 in control digesta, Other treatments were: FOS 1.43, IMO0.81, MOS-Pr 1.75, Probio 0.42 and Antibio 0.83 for near end ileum; and FOS 3.75, IMO 0.61, MOS-Pr 1.57, Probio 1.37 and Antibio 2.75 for caecum. There were not significant difference on tested IgA and IgG levels between treatments, not so were the ratio of lymphocyte T/B and tested IL-2, but IL-2 increased by 16.1% than all others in Antibio.Comparing with control, mucosa and villi heights of near end ileum were significantly (p<0.05) increased in FOS and MOS-Pr, and unsignificantly increased in Antibio, crypt depth significantly increased in MOS-Pr; mucosa and villi heights of nea...