Studies On Factors Affecting In Vitro Fertilization Of Porcine Oocytes

The objective of this study was to investigate the effects of duration of exposure to hormone supplements, maturation media and protein additives on porcine oocyte maturation and subsequent embryonic development after in vitro ferti lization. The results show that: 1) supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free contro I, but cumu I us expans i on i s not necessar i I y re I ated to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2) exposure of pigCOCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturationcompared to that for 46-48 hours; 3) under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4) the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5) different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.