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Study On Mechanism And Inheritance Of Salt Tolerance In Wild Soybean (Glycine Soja) And Cultivated Soybean (G.max)

Posted on:2004-01-29Degree:DoctorType:Dissertation
Country:ChinaCandidate:Q Y LuoFull Text:PDF
GTID:1103360095462317Subject:Botany
Abstract/Summary:PDF Full Text Request
Evaluation, selection and utilization of wild germ plasm to enhance salt tolerance of plants by breeding is very important to the exploitation of saline soil. 6 species of soybean cultivars (Glycine max L.)-Lee68, Nannong 1138-2, Nannong 88-31, No.1 of Suxie, Jackson and Zhongzi Huangdou-Yi and 5 populations of wild soybean (G soja L.)-N23232, N23234, N23227, BB52 (collected from the setuary of the Yellow Rive in Kenli County, Shandong Province) and JWS (collected from the beach in Sheyang County, Jiangsu Province) were used as plant materials in this research. At the first, evaluated for their ability of salt tolerance at various stages of growth and development were evaluated; At the second, effects of NaCl stress on the development and salt secretion of glands on surface of stems and leaves in G max as well as G soja were investigated. At the third, stress effects of Na+ and Cl- caused by NaCl on seed germination, seedlings growth, H+-ATPase activities of plasma membrane, H+-ATPase and H+-PPase activities of tonoplast of the roots and contents of polyamines of the leaves in G max and G soja were compared. Finally, inheritance of NaCl and Cl" tolerance in G max and G soja were investigated. Result showed that:Under NaCl stress, among the 5 G soja populations, seed relative germination index were BB52, JWS>N23232, N23234 and N23227, seed emergence rate were JWS>BB52> N23227>N23232>N23234, salt tolerance at whole development stage were JWS, BB52>N23232. Among the 6 G max cultivars, under NaCl stress, seed relative germination index were Lee68, Jackson, Nannong 88-31>Zhongzi Huangdou-Yi, Nannongll38-2> No.1 of Suxie, seed emergence rate were Zhongzi Huangdou-Yi> Lee68> Jackson, No.l of Suxie>Nannong88-31> Nannongl 138-2, salt tolerance of seedlings were Lee68, Nannong 1138-2>Nannong 88-31> No.l of Suxie, Jackson> Zhongzi Huangdou-Yi, salt tolerance at whole development stage were Lee68, Nannong 1138-2> Zhongzi Huangdou-Yi. These results indicated that salt tolerance of G soja populations at various stages were performance consistence but inconformity for G max cultivars; and the salt tolerance atseedling stage were performance consistence with those of the whole development stage for G max cultivars.The results observed with scanning electron microscope showed that, the globe "glands" were born in surface of stems and the adaxial/abaxial surface of leaves, and mainly attached to the epidermal cells in abaxial surface of leaf apex, near and on the main/later vein and guard cells of stoma. And their development progress was synchronizing with the leaf and stem it attached and their density was promoted by NaCl stress. By the way, the globe "glands" were not monopolist by the seedlings of BB52, and were founded in seedlings of other populations of G. soja and cultivar of G max. The results studied with electro-probe (X-ray microanalysis) and leaf rinsing showed that, under NaCl stress, chemical element constitute in the globe "gland" surface regarding Si as the lord, contents of Na+ and Cl- were very low. The globe "gland" couldn't secrete salt.At seedling emergence stage and seedling stage, the relative germination index and salt injury suffered under isoosmotic stress of Cl-, Na+ and NaCl at equal concentration were used to evaluate and compare the stress effects of Cl" and Na+ on G max cultivars and G soja populations under NaCl stress. Results showed that, cultivars of G max were more sensitive to stress effect from Cl" than that from Na+; however, populations of G. soja were more sensitive to stress effect from Na^ than that from Cl".In the period of seedling, content of polyamines in leaves as well as activities of I-T-ATPase of plasma membrane and activities of H+-PPase and H+-ATPase of toloplast in roots of G soja populations were mensurated under isoosmotic stress of 150 mmol/ L Cl-, Na+ and NaCl. Results showed that, the activities of plasma membrane H+-ATPase, toloplast H+-PPase and H+-ATPase increased after stress for 1 day; after stress for 2 days, their activities decreased to the levels...
Keywords/Search Tags:G max, G soja, Gland, Salt tolerance, Inheritance, Joint segregation analysis of multiple generations
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