Metabolomics Analysis Reveals The Salt Tolerant Mechanism In Glycine Soja

In this study,common wild soybean and salt tolerant wild soybean were used as experimental materials,and these seedlings were cultivated by sandy soil to compare with the changes of metabolites in the leaves of two wild soybean seedlings under neutral-salt stress and alkali-salt stress.The differences in metabolite profiles were analyzed between common wild soybean and salt-tolerant wild soybean in response to neutral-salt stress and alkali-salt stress in order to elucidate the salt-tolerant mechanism.The results showed salt-tolerant wild soybean grew better than common wild soybean under both treatments.Differential metabolites profiling showed that the levels of some carbohydrates and fatty acids were lower in common wild soybean than in salt-tolerant wild soybean under salt stress.These metabolites included lactose,ribose,lauric acid,palmitic acid,stearic acid,and linolenic acid.Amino acid accumulation was observed in the two wild soybeans under alkali-salt stress.These amino acids were valine,tyrosine,glutamic acid,leucine,and isoleucine.The content of most organic acids and proline increased in salt-tolerant wild soybean subjected to alkali-salt stress.These organic acids included mucic acid,glutaric acid,galactonic acid,and dehydroascorbic acid.The TCA cycle was enhanced in common wild soybean in response to both treatments,but was reduced in salt-tolerant wild soybean.This study demonstrated:First,the growth of two wild soybeans were inhibited under both stresses,and more inhibition in roots;Second,two wild soybeans in response to alkaline salt stress is more intense,salt-tolerant wild soybean showed stronger ability to adapt to stress environment;Thirdly,Common wild soybean may encourage the TCA cycle to generate more ATP.However salt-tolerant wild soybean may regulate amino acid and organic acid metabolism to generate more compatible solutes.In the paper,the salt tolerance mechanism in wild soybeans was revealed under salt stress based on metabolomics.It provides important theoretical basis for improving cultivated soybean resistance in adverse environment.This showed that wild soybean is a valuable resource for genetic improvement of cultivated soybean and the development,utilization and protection of wild soybean germplasm resources are great significance.