| Genie male sterility (GMS) is one of the most important ways to utilize the heterosis in Brassica napus and the exploitation and study of the new source of male sterility is always its important basic work. Shaan-GMS was a newly discovered male sterility accession in Brassica napus in 1994, which fertility was very stably expressed in various genetic background and environment In this paper, 83 Brassica napus accessions from China and abroad were tested for their maintaining or restoring ability for Shaan-GMS. Among which, two accessions were screened out with the restoring ability for Shaan-GMS. The genetic pattern of fertility restoration of male sterility of Shaan-GMS was also investigated. Homozygous male sterile line 803AB was developed from the progeny of the cross between Shann-GMS and its restorer 96-803. Near isogenic line 220AB derived from Shaan-GMS was analyzed for the characteristic of its isozymes, protein and RAPD molecular markers. PCR technique was employed to amplify DNA fragment homologous to MS2Bnap gene reported in GeneBank using the genomic DNA of 220AB in rapeseed (Brassica napus} as template. The main results were as following.1) 83 Brassica napus accessions from China and abroad were tested for their maintaining or restoring ability for Shaan-GMS, among which two accessions were screened out with the restoring ability for Shaan-GMS. The genetic pattern of fertility restoration of male sterility of Shaan-GMS was investigated. The results showed that male fertility of Shaan-GMS was controlled by two pair of nuclear genes. If male sterile gene was designated as MS , its allele recessive gene asms, dominant inhibition gene as Rf, which can inhibit the expression of the MS, resulting in the restoration of F1, its allele recessive gene as rf, the phenotype of MS_rfrf was male sterile, and that of other seven genotypes was male fertile .So the genotype of Shaan-GMS, 96-803 and 84004 was Msmsrfrf, msmRfRf and msmsrfrf respectively. The testcrosjs results also showed that Shaan-GMS had similar maintaining and restoring relationship with 6CA,which genotype was Msmsrfrf.2) Dominant genie male sterility (DGMS) Shann-GMS was crossed with its restorer96-803. F2 were obtained by selfing FI. Fertile plants from the fertility-segregating F2 family lines were selfed to obtain Fa. Male sterile plants of the fertility-segregating F3family lines were test-crossed by temporary maintainer 84004(msmsrfrf). Based on the test-crossing results, Sib-mating was made in Fa family lines in which FI of male sterile plants with 84004 were all sterile. Using this method, homozygous male sterile line 803AB(MsMsrfrf X MsMsRfrf) was obtained.3) The zymograms of some isozymes and soluble proteins(Prot) in different size buds and stamens of two DGMS lines,220AB derived from Shaan-GMS and FI (6CAX220) in Brassica napus were analyzed using PAGE (polyacrylamide gel electrophoresis).The results showed that Esterase(EST) had 13 bands, Prot had more than 20 bands, Catalase (CAT) had 8 bands and a -Amylase( a -AMY) had 12 bands. The band number of EST and Prot in the 3-4mm length buds and their stamens were less than those in smaller buds and those in fertile plants, both in 220AB and Fj (6CA X220) .Some bands of EST, Prot, and CAT moved forward and their Rf values increased due to degradation of the molecular. The change of CAT was similar in the two DGMS types. Compared with the control, electrophoresis pattern of a -AMY showed 5 new additional bands in 4-5mm length buds of sterile plants in 220AB, which meant that the starches was degraded and energy was deficient in the stamen.4) Totally 825 random 10-mer primers were screened on the DNA samples of fertile and sterile bulks of 220AB derived from Shaan-GMS and the control Fi(6CA X 220),among which 632 primers produced 2043 bands, One primer named BA1102 produced repeatable polymorphic band BA1102.500 between the paired bulk of 220AB. However, it didn't produce polymorphic band between the paired bulk of Fi(6CAX220). Furthermor...
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