| Fermentative detoxicating method is microbes detoxicating method Of RSM. Microbes can decompose all the natural material with the characters of great quality, many species, fast growing, easy culture and complicatedly enzymes. There were some characters with detoxicating microbes. First, the enzymes were complicated, complicated and changefull GS could be decomposed. Second, the middle matters could be decomposed by the microbes enzymes, too, and selfish material were composed. Third, Phytin acid, Tannins, Sinapine and Fibre in RSM could be decomposed, nutrition and utilization ratio could be raised. Fourth, some fragrant matters were produced with the microbes self-metabolism, and the taste of detoxicated RSM was changed. The test proved that microbes detoxicating method had a great future.By the experiments of detoxicating microbes seporation, purification and detoxication of RSM in laboratory and feeding test within two ywers, the detoxicating microbes abundance and purification of RSM in soils, single detoxicating effect of 40 species of microbes, the detoxicating result of moulds in genus, between genera and microbes distant combination, feeding pigs and Tenebrio molitor with FDMRM-1 detoxicated RSM were studied in this paper. The main results are as following:1. The microbes of RSM detoxicated were abundanced in test, in the A soil, B soil and C soil using RSM as fermentation base, abundancing time 8 weeks, determination every week. B in the A soil increased 4.5 times, in the B soil 4.0 times, in the C soil 3.4 times. M in the A soil increased 10.01 times, in the B soil 11.71 times, in the C soil 11.66 times. A in the A soil increased 1.5 times, in the B soil 2.8 times, in the C soil 3.3 times. Y in the A soil increased 4.0 times, in the B soil 7.5 times, in the C soil 9.0 times. The results of dotoxicating in A soil during the abundance were satisfied. This test proved that action of detoxicating was M >Y > B > A on the basic of microbes change. Microbes of 40 species was sepereted and purified from the abundanced soil with RSM, and that wound be used to study and produce the FDMRM-1.2. There were plenty of structures in RSM toxic GS which exited in common in one sample. The content and constituent of GS were different in different species of RSM and in different RSM sample of a common species because the content and constituent of GS were decided by not only the inherent characteristics of rape but also their cultivated conditions of rape, stored conditions of rapeseed, processed conditions of plant oil and stored conditions of rapeseed meal. It was that the structures of GS were complicated and changeable to the species of RSM.3. There was one type thioglucosidase or more in one detoxicating microbe. It could only decomposed the glucosinolate which structure was correspending to the enzyme in RSM. It could no decomposed the GS with different structure. The highest measure of detoxicated GS in one microbe was the percentage correspending structure GS with total GS. The typies of thioglucosidases were different in different microbes so the GS decomposed rates were different in a common RSM sample. The GS decomposed rate of a common microbe was different in fifferent sample of RSM (there were difference in different RSM sample). In fact the combination between microbes was the combination of GS enzymes. Because the typies of thioglucosidase owing to the combination were raised and theproportion of correspending structure with total GS was increased the detoxicated rate of GS could be raised, the detoxicating rate of GS with M in genus > the detoxicating rate of GS with M between genera. The detoxicating result of many microbes distant combination was sotisfied (99.0%).The content of protein in detoxiated RSM was increased to 39.2% because of the joining of some microbes which could compose their cell protein from inorganic nitrogen and the nutrition value of detoxicated RSM was raised.There was interlock appearance among enzymes between microbes. If one microbes GS enzym...
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