Study On Inducement Of Antimicrobial Peptides Of Tenebrio Molitor And Their Antibacterial Action To Important Aquatic Bacteria

The protein of Tenebrio molitor L. is considered to be a high-quality,safe andsustainable source of high protein in the exploitation and utilization of insect protein.In this paper we research on the inducement to antimicrobial peptides in T. molitorand their inhibitory action to some important aquatic bacterias, including theinfluences of high temperature treating to antimicrobial peptides on the activity ofinhibiting aquatic bacterias, and some basic biological researches on T. molitor is alsoconducted, which will provide theoretical basis for the better use as feed of T. molitorproteins.1.The lify cycle,development duration of different stages and changing regularityof larva weight of T. molitor were tested at28℃and RH70%, and the results showedthat the development duration of different stages are obviously different especially inlarvae and adult. The larva weight changed regularly, which increased slightly beforethe fifth instar larva and appeared a brief decrease between the fifth and the sixthinstar larva. After growing into the fifth instar larva, the weight of larvae increasedmore obviously with linear increase trend, and reached to the highest in the10thinstarlarva.2.T. molitor was induced to produce antimicrobial peptides by using differentmethods and bacteriostatic experiments of important aquatic bacteria were undertook.The results showed that the inducement effect of the methods used were bacteriumfluid injection>ultraviolet radiation>bacteria liquid feeding> ultrasonic treatment,and the inducement effect changed regularly with the change of dose and time. Theantimicrobial peptides produced by the same method had clearly different inhibitiveeffects to several aquatic bacterias used in this paper, as Pseudomonas fluorescens>Vibrio harveyi> Vibrio alginolyticus> Vibrio anguillarum> Vibrio Parahaemolyticus> Vibriovulnificus> Aeromonas hydrophila, in which the effect is the best in inhibitingpseudomonas and the worst in Aeromonas hydrophila.3. Experiments on the heat resistance of antimicrobial peptides produced by T.molitor through ultrasonic treatment showed that antimicrobial peptide was morestable after water heat treatment than dry heat treatment, and the activity ofantimicrobial peptide decreased at80℃in water heat treatment, but in dry heat treatment the activity reduced clearly at70℃.