| Background:Ultraweak biochemiluminescence (UBC) is a widespread phenomenon inliving systems, which is correlated with the many basic life processes, suchas biological oxidative metabolism, defense response, apoptosis, cell agingect. Through the study of it, the information of the physiologic biochemicalprocess in organism can be known.Recently, based on the study of UBC, the biophotonical technique isdeveloped gradually and applied in medicine, foodindustry, agriculture andenvironmental science widely.Aims:This research is to apply the biophotonical technique based on the study ofultraweak biochemiluminescence (UBC) into the agricultural science, tostudy the vitality of rice seeds and stress reaction of the injured plant and tofind a way to determinate it quickly and precisely. This research also makesome explorations in its probable mechanism.Materials and Methods:1) Study of the vitality of rice seeds:With high sensitive single photon counter device made by ourselves, UBC of some rice seeds of different ageing degree (stored in different year and accelerated aging) were measured during their early imbibition period. Then, some reagents (active oxygen scavenging reagents and chemiluminescence probe) were added to the experiment, and the changes of UBC intensity were compared with the former results. Meanwhile, germination test and physiological and biochemical test were carried out to measure the vitality of rice seeds with different ageing degrees. Then, theresults were compared, and the relationship among them was analyzed. 2) Study of stress reaction to injured soybean cotyledon:The changes of UBC were observed with high sensitive single photon counter device in the stress reaction experiments of the soybean cotyledon (etilolation and green) after being injured. Then, some reagents (active oxygen scavenging reagents) were added into the experiments, and the changes of UBC intensity were compared with the former results. Results:1 ) The dry rice seeds exhibited low UBC intensity. But after adding water, the photon emission from seeds during early imbibition was rapidly increased, and then decreased slowly. For the same kind of seed, the lower the ageing degree was, the higher the UBC intensity in the early imbibition was, the higher the germination rate was, and the higher activity of active oxygen scanvenging enzymes was, i.e., the higher the vigour of rice seeds was. With the vigour of seeds decreased, the UBC intensity became weak. The UBC intensity had a high positive correlation with the germination rate (R=0.99)2) All kinds of active oxygen scanvenging reagents could reduce the UBC intensity of rice seeds to some extent. Among them, the inhibition of NaNs was the greatest, which could reduce 30-40% of the UBC intensity.3) MCLA (2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo-[ 1 ,2-a] pyrazin-3-one) could increase obviously the UBC intensity, due to the output of singlet oxygen (1O2) and superoxide anion (O2). NaN3 and SODcould eliminate 1O2 and O2 produced by MCLA-mediated chemiluminescence, which showed that 1O2 played the main role. The output of Singlet oxygen had an obvious positive correlation with the germination rate (R-0.92).4) The UBC intensity of green soybean cotyledon was higher than that of etilolation cotyledon. There were great increases of the UBC intensities after being injured of the both green and etilolation soybean cotyledon. However, the increase of green was much greater than that of etilolation soybean cotyledon. At first, the UBC intensity of injured green cotyledon was very high, and then reached to a stationary state after about 5 to 6 minutes, which was still higher than that of non-injured cotyledon at that time.5) All kinds of active oxygen scanvenging reagents could reduce the UBC intensity of the injured green soybean cotyledon to some extent. Among them, the inhibition of NaNs was the greatest, which could reduce 50% of the UBC intensity.6) D2O could enhance the UBC int... |
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