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Acquirement Of The Ogura Cytoplasmic Male Sterile Lines And Genetic Studies During The Procedure Of Their Creation In Chinese Cabbage-pak-choi (Brassica Campestris Ssp. Chinensis Makino) And Turnip (B. Campestris Ssp. Rapifera Sinsk)

Posted on:2005-12-31Degree:DoctorType:Dissertation
Country:ChinaCandidate:H M CuiFull Text:PDF
GTID:1103360122988025Subject:Vegetable science
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Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino; syn. B. rapa L), originated from China, is one of the vegetable cultivars possesing the largest sowing area among all vegetable crops in China. There is a long history about the cultivation of Chinese cabbage-pak-choi, therefore its resources of cultivars is very affluent. Chinese cabbage-pak-choi and turnip (B. campestris L. ssp. rapifera (Matzg.) Sinsk ) are typical allogamy plants and have obvious heterosis. So far much attention has been paying to the research on breeding of the male steriles line and basis for application in Chinese cabbage pak-choi. In this research the Ogura CMS (cytoplasmic male sterility) lines of Chinese cabbage-pak-choi (B. campestris L. ssp. chinensis Makino var. communis Tsen et Lee) and turnip were obtained through interspecific hybridization between Ogura CMS line in B. napus and Chinese cabbage-pak-choi and turnip, and successive backcrosses with Chinese cabbage-pak-choi and turnip as male parents. In the course of acquiring cytoplasmic male sterility lines, Studies were performed on morphology, cytology, physiology, biochemical and molecular biology in various backcross generations. The main results are as follow:(1) Comparative analysis has been made among backcross generations and their maintainer lines on field growth condition. The results of plant morphology in field indicated that three F1 plants had the same morphological and sterile properties, which similar to B. napus with strong growth. There were mainly two morphologically type in BC1 plants. One type had strong growth similar to B. napus, the other type had weak growth similar to the parent of backcross. Male sterility occurred in all the plants of the BC, generations. Some lines in 'Xinxuan 992-24' and 'Aijiaohuang 991-6" BC2 generation had the same growing characteristics as their recurrent parents, while the BC2 plants of 'Naibing 98-1' were phenotypically intermediate or similar to the B. napus parent. In 'Naibing 98-1" BC3 generation plants had the same morphological character as their recurrent parents. The 'OguXX 00-4-7A' , 'OguAiH 00-14-17A' and 'Ogu NB 00-1-1-1 A' CMS lines and 'OguXX 00-4-7B' , 'OguAJH 00-14-17B' and 'Ogu NB 00-1-1-lB' maintenance lines were obtained. The CMS lines seedlings did not show chlorosis at low temperature. All the plants of the CMS lines had normal nectary and male gametes were highly sterile and female gametes were fertile. F1 obtained from 'OguXX 00-4-7A' , 'OguAJH 00-14- 17A' as female parents, and 'ZHB012-08' , 'AKQ011-16' , 'HYC025-44' and ' J7809-011-15' as male parents respectively. The results indicated that three F1 have heterosis, andthe CMS 'OguXX00-4-7A' and 'OguAJH 00-14-17A' could be used to produce F1 hybrids.(2) The chromosome meiotic behavior of F1 hybrids and their backcross generations between B. napus L. CMS line and B. campestris L. have been analyzed. The chromosome configuration of F1 hybrid were 10II+9 I . In the plants with strong growth plant in BCi, the chromosome configurations of 'Xinxuan 992-24' and 'Naibing 98-1' were 10 II+9 I , and the chromosome configurations of 'Aijiaohuang 991-6' were mostly 10II or 10II+7 I . In the plants with weak growth in BC1, the chromosome configuration of 'Xinxuan 992-24' and 'Naibing 98-1'were 10 II +7 I , and the chromosome configurations of 'Aijiaohuang 991-6' were mostly 10II+1 I . In the plants with strong growth plant in BC2, the chromosome configurations of'Xinxuan 992-24' and 'Aijiaohuang 991-6' were 10II+1 I and 10II, respectively, and the chromosome configurations of 'Aijiaohuang 991-6' were mostly 10II. In the plants with weak growth in BC2, the chromosome configurations of 'Xinxuan 992-24' and 'Aijiaohuang 991-6' were 10II, and the chromosome configurations of Naibing98-1 were mostly 10II +6 I and 10lI+5.The chromosome number in BC3 generation of 'Aijiaohuang 991-6' and 'Xinxuan 992-24' were 20. The percentage of cell with 2n=20 was 78.12% in BC3 generation of 'Naibing 98-1'. These results sugges...
Keywords/Search Tags:Chinese cabbage, turnip, Brassica campestris, Brassica rapa, Ogura cytoplasmic male sterility, microsporogenesis, chromosome, meiosis, physiological and biochemistry, cDNA-AFLP, orf138, gene cloning
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