| Non-heading Chinese cabbage (Brassica rapa ssp.chinensis) CMS line S2108A, Y3611A and its maintainer line S2108B and Y3611B as experimental materials, Cytology&Combining Ability and Molecular marker research were conducted. The results are showed as flows:1Morphological studies on flower organ of Ogura cytoplasmic male sterile and its cytological characterization of abortion in non-heading Chinese cabbageThe results showed that there were significant differences in several characters of blossom organ between two set of male sterile lines and their maintainer lines, while between the two male sterile lines S2108A and Y3611A, the difference was not found except in the length of flower bud pistil and length of petal, suggesting that no significant difference in blossom morphology between the two male sterile lines transferred from different origin. The anther abortion of the two male sterile lines started from tetrad period, that due to taptum’s vacuole changing and radial expandation, extrusion tetrad microspore and result in fail development, indicating the anther abortion had relationship with taptum abnormally development. Moreover, different from Y3611A, there is another anther abortion style in S2108A, taptum cell wall is fused, turn into periplasmodium like amoeboid tapetum cling to powder chamber wall, part of cytoplasm afflux into powder chamber, wrap and conglutinate tetrad microspore, the anther abortion. The number of clinandrium of the two male sterile lines is different, and development period of clinandrium is asynchrony, some clinandrium is late. In addition, huge clinandrium is found in the line of Y3611A.2Combining ability and heterosis analysis with Ogura cytoplasmic male sterility in non-heading Chinese cabbage Two non-heading Chinese cabbage male sterile line S2108A (B1) and Y3611A (B3) and its corresponding maintainer line S2108B (B2) and Y3611B (B4) as the female parent, five high-generation inbred lines (A1～A5) as the male parent, hybrid were carry out. Heterosis, specific combining ability (SCA) of those hybrid combinations were determined in eight traits aspects of height of plant, expansion of plant, length of the largest leave, and width of leave, length of petiole, width of petiole, leaf number and single plant weight, as well as the general combining ability effect of the nine parent materials. The results showed that:Heterosis (positive or negative) of non-heading Chinese cabbage was obvious in horticultural traits; there is no difference in performance due to the female parent heterosis effect of length of leave and length of petiole; in height of plant and other traits, combinations of the maintainer line have a greater positive effect, while the CMS combinations have a greater negative effect; leaf number, width of petiole and single plant weight have a negative effect in several combinations; length of petiole and height of plant have a positive effect in several combinations; GCA effects of female parent B1, B2are mainly negative, while B3, B4have positive effect.3AFLP analysis associated with Ogura cytoplasmic male sterility in non-heading Chinese cabbageAFLP with64pairs primer combinations Pst I-NNN+Mse I-NNN was employed to find the polymorphisms between the genomic DNAs of CMS line Y3611A and its maintainer line Y3611B in non-heading Chinese cabbage.5185bands were amplified in two lines with61pairs of primer combinations, including3specific amplified fragments of CMS line using primer combinations AAG/CGC、AAC/GAC and ACA/GAC. Further more, these specific AFLP fragments were cloned and sequenced. The lengths of these fragments were187bp,205bp and237bp, respectively, and were denominated CMS187, CMS205and CMS237. BLAST analysis of its nucleotide sequence found a high homology with the portion of nuclear genome of Chinese cabbage, senescence-associated protein mRNA and mitochondrial genome DNA of Arabidopsis thaliana. |
PDF Full Text Request