| In these studies, mature sows and female mature rats were regarded as experimental animals and that techniques such as gel chromatography and immunoaffinity chromatography with magnetic beads, chlorine sign, Blood-bra in barrier (BBB) transport, filling in vitro, autoradiograph, immunohistochemistry, fluorescenthistochemistry and affinity chromatography were respectively used to purify porcine inhibin, to sign inhibin with iodine, to study whether inhibin transport through Blood-brain barrier, and the location of inhibin and the inhibin receptor , finally to purify and identify the inhibin receptor. The main content presents as follows:1 The purification of porcine follicular inhibin and the sign of itThe porcine follicular fluid was collected and treated with 70% ammonium sulphate to precipitate proteins. Inhibin content in the precipitate was examined by ELISA. The precipitate was passed through columns fitted with Sephadex G - 200 (100 cm X 2. 6 cm ) firstly and then with Sephadex G - 100 (30 cm X 1.6 cm), and finally by chromatography with magnetic beads to purify inhibin. Purification of inhibin increased from 12.4 ug. mg-1 to 39. 4ug. mg-1. PAGE showed that there was only one zone with molecular weight of 32, 000 in pure product. By SDS - PAGE, two bands with molecular weight of 14, 000 - 15, 000 and 17, 000 - 18, 000 were observed, which presented a - and B -subunits of inhibin molecule, as a result, pure inhibin was obtained.Iodine was used to sign inhibin with mild chlorine because the activity of inhibin was easy to lose. The results showed that the radiochemical pure degree was more than 95%, and that the signing rate, radiative intension , the rate of radioactivity and mass radiative concentration were respectively 85%, 7.04X103 Bq. s-1, 4.3 uci.ug-1 and 0.2 uci.mL-1.2 Inhibin transport by the brain microvessel endothelial cellThis study included two parts, one was vesicle transporting in vitro, and the other was filling in vivo.In vitro experiment, sex mature erhualian sows and Landrace sows were selected as experimental animal. Firstly, the BBB was prepared and identified. The micro - vesicles were isolated and purified from the endothelial membrane of micro- vessels on cerebral cortex of 5 for each Erhualian and Landrace sows. The integrity of the vesicles was evaluated by the inverted microscopy and the electron microscopy. The purity and biological activity were determined by the activity of such enzymes as y - glutamy transpeptidase (y - GTase), 5' - nucleotidase (NTDase), acetylcholinesterase (ACSase), lactate dehydrogenase (LDHase) and alkaline phosphatase (AKPase). The results indicated that the endothelial membrane vesicles isolated by the above - mentioned method was of high purity and biological activity and was a useful blood - brain barrier model for transport of the BBB. So it was applied to transport of follicular inhibin by method in conditions of suitable vesicle concentration, reaction time, ion chunnel, osmosis etc. The results showed that follicular inhibin could be transported by vesicles from the blood - brain barrier of sows. The Km values of transport in Erhualian and Landrace breeds were 0. 17 pmol.L-1 and 0. 02 pmol. L-1, respectively. The Vmax values of transport were 1.15 pmol. min-1. mg-1 and 2.27 pmol. min-1.mg-1.In filling experiment, sex mature female SD rats were selected as experimental animals. 20 rats were allotted as four goups, five rats one group. NO 1, NO 2, NO 3 groups were experimental group, and NO 4 group was control group. Rats were anaesthetized by 10% Urethane before experiment. The rats of the experimental were injected 50 ul 125I- INH through jugular vein, and the same amount of saline was injected into the rats of the control goup. Rats of NO 1, NO 2, NO 3 groups were respectively killed 30 min, 60 min and 120 min later. Immediately, the radiative intension of the pituitary and the hypothalami were counted for 1 min in y- geodesic machine . The pituitary with the highest radiative intension and the corresponding hypothalami and those of co...
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