Effects Of Environment Factors On Osmoregulation And Immune Parameters Of Crustaceans

The paper used Litopenaeus vannamei, Marsupenaeus japonicus and Eriocheir sinensis as study objects, and researched the effects of environmental factors on hemolymph osmotic press, ion content, gill Na+-K+-ATPase activity, haemocyte count, phenoloxidase activity, bacteriolytic activity and antibacterial activity of Crustacea. It discussed the environmental physiology mechanism of crustacean osmoregulation. It not only demonstrated the physiology adaptation and regulation rule of Crustacea when environment factors changed, but also had innovation at the research methods. It established a theory foundation of crustacean osmotic and immunological physiology, and also provided a scientific basis for water circumstance regulation and disease prevention and cure in the prawn healthy aquaculture.1 The Effect of Salinity and pH on Gill Na+-K+-ATPase Activity of Li topenaeus VannameiThe results indicate that salinity and pH changes have a notable effect on gill Na+-K+-Atpase activity of Litopenaeus Vannamei (F>F0.01). During the first 3 days of the salinity treating, the gill Na+-K+-ATPase activity of the shrimp in all treatments increase gradually with the elapse of time, and the enzyme activity in every treatment tends to be stable in the period from the third to fifteenth day and are markedly high as compared to thecontrol treatments (F>F0.05) , meanwhile the salinity lowers as the enzyme activity increases. During the first 3 days of the pH changes, the gill Na+-K+-ATPase activity of all treatments changed in curvel loci, and there peaks appeared at 9th hour and 12th hour separately when its changes were decreasing or increasing. During the period from the third to twelfth day, gill Na+-K+-ATpase activity of all treatments tends to be stable and have no significant differences (F