Transformation Of Herbicide Resistant Bar Gene Into Wheat And Maize By Agrolistic And Three Nontissue-culture Methods

Biotechnology has provided new avenues for stress tolerance andresistance breeding of crops.bar gene was introduced into wheat immature embryos (10-15 daysafter pollination) by Agrobacterium tumefaciens mediated transformation.PPT(5 ‰) selection and further PCR and PCR-Southern blot testdemonstrated that bar gene has been integrated into plant genome. Themajor procedures for obtaining high frequency plant regeneration werealso discussed.A new procedure of Agrobacterium-mediated transformation wasused to transform germinating seeds. First, maize seeds were submergedin warm water for 4 hours, then a wound was made in appearing sproutsof the seed and the wounded germinating seeds were cocultured with theAgrobacterium suspension (containing AS) on a shaker for 24 hours.Transgenic plants were selected by basta spraying (5‰) and verified byPCR and PCR-Southern blotting. It was proven that the approach notonly simplified the plant transformation technique by circumventingtissue culture procedures, but also produced high transgenic rate.The three nontissue-culture plant transgenic methods: pollenmediation, Agrobacterium-mediation of germinating seeds, and ovary...