Study On Preparation And Application Of Molecularly Imprinted Solid-Phase Extraction Cartridge For Chloramphenicol

Chloramphenicol (CAP), the prohibited veterinary drug, is emphasized on its detection and supervision by all countries. In the procedure of detection of CAP, the low-selective and nonspecific solid-phase extraction (SPE) cartridge is popularly used, but its purification is not very effective, so the sensitivity of the detection for CAP is decreased. For improving the purification effect and increasing the sensitivity, in this dissertation, the detection method for CAP is improved, and the molecularly imprinted SPE (MISPE) cartridge for CAP is prepared; through its application in detection of CAP or CAPs in bio-samples and animal original food, the problem in the detection of CAPs is resolved.The main results are as follows,1. The method for determination and confirmation of Chloramphenicol, Thiamphenicol (TAP) and Florfenicol (FF) is developed, and the micro-pretreatment is introduced. The method simplifies procedures, reduces consumption of organic reagents, and shortens the detection time. The detection limit is 0.01μg/kg and the lowest level all over the world now. At the same time, this method is the first time to detect and verify CAP, TAP and FF simultaneously. As to the more successful detection methods by GC/ECD and GC/MS/NCI, the necessary validation is performed.2. In the preparation of molecularly imprinted polymers (MIPs), the molar ratios of template to monomer and cross-linker, porogen selection, and polymerization temperature all influence the binding characteristic of MIPs. As to the preparation of molecularly imprinted polymer from methacrylic acid, the optimal condition is as follows: the ratio of template to monomer and cross-linker is 1:2:20; tetrahydrofuran is taken as porogen and polymerization temperature is 50℃.3. The study on morphology shows, different MIPs have different apparent dry density, swelling, thermo-stability and external modality and structure; there are different quantities of free carboxylic acid groups in MIPs, and the one in P₁ is more than that in others; more micro-pores and less surface area in P₁ than those in P₀; thermo-treatment of 120℃ has almost no effect on pore structure, but thermo-treatment of 180℃ causes serious destroying.4. The study on absorption kinetics shows that the selective binding is stronger than non-selective absorption in P₁; in order to study the binding characteristic of P₁...