Studies On Preparation Of Organoarsenic Veterinary Drug Molecularly Imprinted Monolithic Column And Its Selective Enrichment And Separation Characteristics

Molecular Imprinting Technology (MIT) has been proved to be an effective tool for solid-phase extraction, chiral separation, sensors, etc. The molecularly imprinted monolithic columns, which combine the high stereo selectivity of molecularly imprinted polymers with the advantages of good hydrodynamic characteristic, low flow resistance as well as easy preparation of monolithic materials.In this thesis, A molecularly imprinting polymeric monolith (MIPM) was prepared by in-situ polymerization using arsanilic acid, an organoarsenic compounds used as an additive to poultry and hog feeding, as template molecules. The imprinting and recognition mechanism were studied and the influence of polymerized conditions on the chromatographic performance was systematically investigated and optimized. Meanwhile, studied its selective enrichment and recognized characteristics and also using MIPs as solid-phase extraction, achieved off-line and on-line selectively enrichment. There are four chapters in this thesis.Chapter one: The principle of MIT, polymerization methods, application of this technique and its future developments were introduced. And the development in analysis technology of veterinary drug residues was also described.Chapter two: Molecularly imprinted monolithic columns based on arsanilic acid and roxarsone respectively were prepared and have been used as HPLC stationary phases. Systematically investigating the effect of polymerization conditions and the recognition mechanism was deduced. Roxarsone has a nitrite groups -NO2 and adjacent group -OH, which can form strong intramolecular hydrogen bond with six-membered ring, thus there are no specific binding sites.