Response Mechanism And Effect Of Some Genes Expression On Cadmium And Lead Ions Stress In Creeping Bent And Perennial Ryegrass

Heavy-metal ions stress was a one of adverse condition for plant grow, the metal ions accumulated by heavy metal pollutant in soil had been badly menaced agricultural environment. How to reduce the harm of the heavy metal ions on plant growth and metabolize, it had been a study focus at environment science and ecology. Because of some herbage plants and turfgrass could abate the heavy-metal ions and could grow in heavy-metals pollutant soil, it was significance to study of the toxicological effect of the metal ions on these plants, and the response mechanism and effect on some expression of the metal ions in these plants.The infancy creeping bent (Agrostis stolonifers ) and perennial ryegrass (Lolium perenne) had been cultivated in Hoagland culture medium by using tank farming. When dealing the infancy lawn with heavy metal ions stress, the different concentration cadmium nitrate and lead acetate were mixed into the Hoagland culture medium. The identical biomass method was used to weigh in the biomass-production of the two species of plant. The fluorescence spectrophotometry method with testing dichlorofluorescein (DCF) in the leaf blade was conducted to determine the effect of the opposite contents of hydrogen peroxide (H₂O₂), a special reactive oxygen species (ROS) in leaf cells of the turfgrass. The photochemical NBT method, the potassium permanganate stactometer and guaiene...