Application Of Morphological And Microsatellite Markers In Selective Breeding Of Fenneropenaeus Chinensis

Developing the new breeding stock and improving the genetic selecting progress are the hinges of the development of the enhancement program for Chinese shrimp (Fenneropenaeus chinensis).Morphological markers are the traditional markers that have been extensively used for a long time for their facility and identifiability. The microsatellite DNA markers have several advantages over other molecular markers. For example, microsatellites allow the identification of many alleles at a single locus; they are abundant and evenly distributed all over the genome; they are co-dominant; little DNA is required and the analysis can be semi-automated and performed without the need of radioactivity. They have been the focus of recent studies on genetic linkage maps, genetic diversity, relatedness determination and QTL locating, etc. They are regarded as one of the most potential and powerful DNA markers even when the studies on their origin and evolvement are not very perfect. In this dissertation, with the morphological markers and microsatellite DNA markers, we try to solve several difficulties met during the genetic selecting program of the Chinese shrimp. The results are as following:1. The body weight, body length, total length, carapace length, carapace width, first abdominal segment height, fifth abdominal segment height, sixth abdominal segment length, number of upper frontal eminence spine and number of lower frontal eminence spine were measured for Fenneropenaeus chinensis. The correlation coefficients among the attributes were calculated. Path analysis was conducted for the body weight and other nine morphometric attributes. The results showed that the carapace width weighted the most to the body weight and was a key effective factor. The attributes with insignificant direct effect on the body weight were neglected in the multiple regression equation. The multiple regression equation of body length, total...