Study On Tachyplesin I Tansfer Into Porphyra Haitanensis

The three contents involved in Porphyra haitanensis transgenic breeding were studied systemically: P. haitanensis transgenic receptor system; GUS gene transient expression within the P. haitanensis thallus somatic cells; The research of tachyplesin I transfer into P. haitanensis cells. Results of the study as below.(1)Study on P. haitanensis transgenic receptor system. Firstly, special activities of three enzymes of cell wall lytic enzymes for seaweed from three species of Gastropoda were compared, the result indicates that Chlorostoma argyrostoma and Lunella coronata coreensis are more suitable as sources of cell wall lytic enzymes for seaweed for red algae as P. haitanensis than Haliotis diversicolor; Secondly, result shows that cell density, illumination intensity and specific gravity have a significant effect on the development and differentiation of P. haitanensis thallus somatic cells; Thirdly, result shows that P. haitanensis thallus somatic cells are sensitive to chloromycetin, this indicates that chloromycetin is a effective seltive pressure for transgenic P. haitanensis.(2)Study on gus transient expression within P. haitanensis thallus somatic cells. Plasmid pBI221 was transformed into thallus somatic cells with the method of electroporation, result of histochemical assay shows that gus expressed transiently within the cells cultured for 72 h and 6 d, expression efficiency were 1.1×10-6 and 1.3×10-6 respectively; Result of quantitative enzyme assay shows that special activities of GUS extracted from transformed cells cultured for 2 d-5 d were all higher than those of controls significantly, this indicates that gus expressed transiently. The results above indicate that exogenous gene transformation system of P. haitanensis thallus somatic cells is effective.