Genetic Relationships Between Pollination Constant Non-astringent Persimmons And Some Staminate Germplasm Native To China Based On RAPD And ISSR Analysis

There are abundant valuable persimmon (Diospyros kaki Thunb.) genetic resources existing in China because of the genetic variation, natural and artificial selection occurring during the long history of cultivation. In the present study, we investigated the phylogenetic relationships among some staminate Diospyros spp. and its relatives using RAPD and ISSR, as while we examined the pollen germinability and the occurrence of giant pollen in the new discovering staminate persimmon germplasm distributed in Dabie mountains. The technique of ITS-PCR and AFLP in Diospyros were also established and optimized. The study on the phylogentics of Diospyros may provide useful information for the analysis of the genetic diversity and molecular systematics in Diospyros as while as the evaluation of the staminate germplasm effects in the evolution of Diospyros kaki Thunb.. The main results were as follows:1. 12 Diospyros spp. staminate germplasm materials were used to test themselves' pollen germinability in vitro and which on the stigma of D.kaki Thunb. cv. Luotiantianshi and the occurrence of giant pollen. The results showed that the pollen germinability in vitro of those materials varied from 20.1% to 38.5%. 10 materials except 'Taiwanzhengshi' (D.kaki Thunb.) and 'Oily persimmon' (D. oleifera Cheng.) had germinability on the stigma of'Luotiantianshi'. There are different percentage of giant pollen existing in 9 germplasm genotypes with variation 0.18% to 4.44% but Male strain No.2,Male strain No.3 and Male strain No.8.2. A total of 499 bands generated by 32 informative RAPD primers screened from 118 RAPD primer, 26 of which were unique to specific accessions in 29 Diospyros spp. The polymorphic rate of RAPD markers was 87.17% and the polymorphic information content was 0.6097. The results of clustering analysis using UPGMA, ME and Wanger Parsimony showed that the 29 genotypes could be divided into 3 groups. The first group included the Chinese persimmon germplasm, while the second consisted of the Japanese persimmon cultivars. Diospyros lotus L. and Diospyros oleifera Cheng. formed the third group. PCNA couldn't be separated from...