Breeding And Charcterization Of Wheat-Thinopyrum Intermedium Alien Substituion Line And Its Genetics Analysis

Thinopyrum intermedium (E1E1E2E2StSt,2n=6x=42) is the third gene pool for wheat, conferring many valuable genes for wheat, and has been widely used in wheat genetic improvement. A novel wheat germplasm, Shannong 0095, was developed from BC2F4 , the self-crossing progenies of common wheat Yannong15 backcrossed two times with hybrid F1 between Yannong 15 and Thinopyrum intermedium, and was characterized by using of cytological genetics, A-PAGE, SDS-PAGE, RAPD, SSR, and GISH before its major agronomic traits were identified. Homoeologous group of alien chromosome in Shanong 0095 was identified, its genetic laws in different wheat background were observed, and its genetic effects on different yield compmonent traits of hybrids F1were statistically analyzed. The two univalents of F1 between Shannong 0095 and common wheat at PMC MI and chromosomes in root cell of Shannong 0095 were micro-dissected by using of CellCut? laser micro-dissection machine. Several new germplasms derived from the progenies of common wheat Yannong15 and Thinopyrum intermedium were identified. The main results were as follows:1. Major agronomic traits of Shanong 0095 were identified. The results showed, comparison with Yannong 15, that Shannong 0095 has stronger stem, longer spike, much more spikelets, more luxuriant than that of Yannong15, which indicated that major genes controlling yield characteristics relative, derived from Thinopyrum intermedium, has been transferred into wheat background.Cytogenetics results showed that the chromosome number of Shannong 0095 in root tip cell was 2n=42 and its chromosome configuration at PMC MI was 2n=21â…¡. The mean chromosome configuration of hybrids F1 of the crossing between Shannong 0095 and Yannong 15 at PMC MI was 2n=20â…¡+2â… . The result of GISH with the probe of Thinopyrum intermedium genomic DNA and the blocker of Chinese Spring (C.S) genomic DNA, further conformed that Shannong 0095 contained 2 chromosomes of Thinopyrum intermedium. The results proved that Shannong 0095 was a wheat- Thinopyrum...