Cloning And Expression Analysis Of Expanding Growth Related Genes From Cucumber Fruit After Pollination

Fruit developmental stages include cell division period, fruit expanding growth period, and fruit ripeness period. The fruit expanding growth is correlated with the economic yield. Naturally, growth and development of plant ovary without parthenocarpy are started only after pollination, otherwise the ovary is shed off. A great deal of research indicated that hormones participated the process of fruit expanding growth and played an important role. The extra-hormones have been widely used to induce ovary without pollination into fruit. Experiments had been done on natural parthenocarpy and induced parthenocarpy with hormones using molecular biology means, but only a few fruit development-related genes have been cloned, and it is not known that which genes inspire or be corrected with the fruit expanding growth. So a lot of experiments need to be done.Cucumber (Cucumis sativus L.) is the good material for the study fruit development because of its ovary character and growth rate. In this work, differential expression of genes in ovary 0 h of cucumber before pollination and young fruit 6~72 h after pollination was investigated via cDNA amplified fragment length polymorphism (cDNA-AFLP). There are 64 transcript-derived fragments(TDFs) displayed specially in young fruit 6~72 h after pollination, Five of which were verified to be mainly expressed in young fruits after pollination by reverse Northern dot blotting. One TDF was a new gene encoding expansin and was named as Cs-Expansin10 (CsEXP10), which minght be related to the expanding growth of cucumber fruit after pollination. Basing on the CsEXP10 gene fragment, a 1191 bp cDNA sequence was obtained via rapid amplification of cDNA ends (RACE) and getting spliced with an EST (AF319471) of the same gene. The bioinformatical characteristics of CsEXP10 were studied using online server and biosoft, including sequence analysis of nucleotide and deduced amino acid, structure and function of CsEXP10 protein. Southern blotting analysis showed that CsEXP10 was a single copy gene in cucumber. The transcripts of...