Analysis Of Maize R.solani AG1-IA-induced Genes Differential Expression

Maize banded leaf and sheath blight (BLSB), caused by Rhizoctonia solani Kuhn, is a destructive disease that results in significant yield loss in most maize-growing areas in China. Although this is a regional maize disease mainly occurring in China and Southeastern Asia, it is possible that this disease may spread to other parts of the world in the future. To breed new varieties with efficient, broad-spectrum, stable resistance to the maize BLSB, the understanding of resistance mechanism is of great importance. In our research, several techniques were used such as electronic micrograph detection, cDNA library construction of suppression subtractive hybridization (SSH), reverse-Northern hybridization, RT-PCR and bioinfonnatics methods. The objective of the present research was to explore efficient methods to analyze high-throughout gene expression, and to obtain a primary gene expression profiling of the disease resistance in maize, and to understand the resistance mechanism against maize BLSB at global transcriptional level.By use of SSH, a pathogen inducible expressing gene library was obtained from high tolerance maize inbreed lines of R15 and high pathogenic fungus anastomosis groups AG1-IA of Rhizoctonia solani Ktihn in Southwestern of China. At the jointing stage, two wheat seeds colonized by AG1-IA were placed into the third sheath of the maize plants. At 12 hrs, 24 hrs, 36 hrs, 48 hrs, 60 hrs, 72 hrs, 84 hrs and 96 hrs of the post-inoculation, the third sheath was picked down to detect the pathology varyment of the mycelium with the electronic micrograph methods. Total RNA from the inoculation and non-inoculation leaves were extract to construct the SSH library. The obtained main results were as follows:1. At the different times of the post-inoculation, the mycelium was changed significantly. Following the inoculations time, the mycelium of the pathogen formed infection cushions, inoculums hoops and single appresoria. Infection pegs arose at the base of these cushions penetrated the cuticle. Also, the pathogenic penetrate through stomata without producing any infection structure at the time of 24 hrs, 36 hrs and 48 hrs after the inoculations. Further penetration was inter or intracellular after the 72 hrs of the...