Molecular Cloning Of Chicken IL-2,IL-15,IL-18 And IFN-γ And The Construction Of Eukaruotic Expression Plamids And Their Adjuvant Effects On Vaccines

chicken interleukin-2(ChIL-2), ChIL-15, ChIL-18 and Chinterferon-γ (ChlFN-γ) play an important role in chicken disease resistance and immune response. Shandi Wugu chicken(SDWG chicken) is a Local chicken breed it had the character of high growth rate, abroad adaptability and resistance to disease.There were few report on Shandi Wugu chicken IL-2, IL~15, IL-18, IFN-γ and their immunity. Cloning of SDWG chicken IL-2, IL-15, IL-18 , IFN-γ, development of melocular adjuvant and study on the immunenhancemnet for vaccines have great academic value and application prospects.1.SDWG chicken IL-2 and IFN- γ was amplified by recerse transcription-polymerase chain reaction(RT-PCR)from peripheral blood lymphocytes total RNA stimulated with ConA. Then cloned into PMD18-T vector and sequenced. The result showed that the open reading frame of IL-2 was 432bp. Sharing98. 4-99. 5% homology with the published. The open reading frame of IFN-γ consisted of 495bp, Sharing97. 4-100% homology with the published IFN-γ gene .There is (few) report about the chIL-2 and ChIFN- γ of SDWG chicken. The gene of chIL-2 and ChIFN-γ in our experiment enriched the banks and provided the material for further study.2. Shandi Wugu ChIL-15 was amplified by RT-PCR from spleen lymphocytes total RNA stimulated with ConA. Then cloned into PMD18-T vector and sequenced. The result showed that the open reading frame of IL-15 was 564bp,...