Effects Of The Recombinant Eukaryotic Expression Plasmid PcDNA3.1(+)-ChIFN-α To The Immunological Function In Chickens

According to the sequences published as accession numbers of EU367971in NCBI, designed a pair of primers of Chicken IFN-a and cloned the ChLFN-a genes.The results showed it was cloned successfully by Double enzyme digestion、PCR identification and sequence anaylsis. comparied witn ChIFN-α genes sequence of NCBI, homology is100%. The pcDNA3.1-ChIFN-α plasmids is successfully constructed through T4DNA ligase connection the two parts which both of the eukaryotic vector pcDNA3.1(+) and pMD19-T-ChIFN-α were digested by ECOR Ⅰ and Hind Ⅲ. chickens were divided into6groups in the immunopotentiation experiment.They were normal sailine control group, vaccine group, empty vector group,3different doses of pcDNA3.1(+)-ChIFN-α adjuvant group by50αg、100μg and200μg. detect the effect of pcDNA3.1(+)-ChIFN-α on the number of activated T/B lymphocytes and antibody levels induced by NDV、IBDand IBV vaccine, the coefficient of spleen, and the protection of chicken under virulent NIBV chicks attacked. The results demonstrated that ChIFN-α has the ability to act as a potent adjuvant in DNA vaccines, increase the levels of T/B lymphocytes in the peripheral blood, promote antibody levels induced by NDV, IBD, NIBV vaccines, increase spleen weight, and protect chickens with NIBV vaccine against strong NIBV attack,The rate of protection increased10percents than the vaccine control group. The dose of100μg pcDNA3.1(+)-CHIFN-α has more obvious effection. This result showed that ChIFN-α has the ability to enhance the immune responses of chickens and act as a potent adjuvant in vaccine.