Damnification Of Heat Stress On Reproductive Function Of The Male Mice

Male Kunming mice were exposed to heat stress within three groups at the temperature same as scrotum (33℃), abdomen (37℃)and severe high temperature (42℃), and the sustaining systemic heat stressed animal model were established by this way. Each group (8d, 13d, 21d and 35d) were divided into four groups with different duration of heat stress according to the phases of the process of spermatogenesis. The changes in the weight and rectal temperature were registered during the process of heat stress. The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination. The methods of immunohistochemistry and western blot were used to study the expression of heat shock protein 70(HSP70); Colorimetry was used to determine the changes in the activity and concentration of active oxygen (ROS), malondialdehyde (MDA) and antioxidized enzymes (SOD, GSH-Px, CAT); Experments of mating were used to determine the reproductive performance of female mice mated by heat stressed male mice and the fertilizing ability of the male mice. Finally, the effects of heat stress on the reproductive function of the male mice were studied systemically. And the results show as follow:1.The changes in the body weight and rectal temperature of the mice before and after the heat stress.Changes of the body weight: the body weight of mice declined before and after the heat stress, and the different value in the body weight before and after the heat stress increased with the treated temperature and decreased with the number of the heat stress.Changes in the rectal temperature: the rectal temperature of the mice rose after heat stress and the temperature increased with the treated temperature. However, there was no correlation with the number of the heat stress. The rectal temperature of the mice returned to the normal level before the next heat stress.2.The changes in the body weight, testicle and the weight exponentialThe body weight, testicle and epididymal weight of the mice after the heat stresss as compared with that of the control group and there was a negative correlation between the above declines and the treated temperature. Except the group of 37℃and 33℃treated for 8d and 13d all the rest groups of the treated mice had a significant (P<0.05) lower body weight compared with the control group;The testicle weights of the mice were significant (P<0.05) lower than the control group, except in the group of 33℃treated for 13d and 21d and 37℃treated for 13d;The epididymal weights of the mice were significant (P<0.05) lower than the control group except 33℃and 37℃...