| Vibrio anguillarum is a major pathogen threatening the fish aquaculture in China. This study investigates the involvement of SNAREs and potassium channel in immune defensive mechanism of turbot(Scophthalmus maximus) after V. anguillarum infection by molecular biological and immunological methods, and patch clamp technique. Fishes were infected with V. anguillarum by injection of a sublethal dose and a cascade of induced response in primary phase of infection were examined. The level of immune response was detected by measuring the phagocytic, bactericidal activity, respiratory burst activity of fishes leukocytes in peripheral blood, spleen and head-kidney and the production of proinflammatory cytokine, interleukin-1βin fish serum. These immune responses consist of innate immune response mechanism in fishes and play an important role in resisting V. anguillarum invasion. The results show that 24 hours after infection with V. anguillarum, the phagocytic percentage of V. anguillarum and the phagocytic index reached to 61.18% and 5.62 , while the control group were 47.25% and 4.68 respectively; The bactericidal index were 1.73, while the control group were 1.26; The respiratory burst activity OD600 increased from 0.07 to 0.2 and respiratory burst activity in spleen leukocytes is the highest compared with head-kidney and blood and the average of OD600 is about 1.7 after 8 hours infection. IL-1βprotein levels in the serum was 0.0523±0.014 at 0 hour and had a progressive increase until reached a maximal level of 0.47±0.033 at 24 hours post-infection. The maximal level at 24 hours was 9-fold that of uninfected controls, the increase was very remarkable (p<0.05). The... |
PDF Full Text Request