| 1. Study on the isozymes of four clam species in Veneridae (Mollusca: Bivalvia)The isozymes (EST, MDH and SOD) from four different tissues (liver, gill, adductor muscle, mantle) of four clams species (Meretrix meretrix; Cyclina sinensis; Ruditapes Philippinarum and Mercenaria mercenaria) in Veneridae were analyzed by vertical polyacrylamide gel electrophoresis (PAGE). The result showed that there was obvious diversity in the isozyme phenotypes among four clams in Veneridae in all tissues inspected, and among four tissues in same species. EST and MDH showed more electrophoresis patterns, so they could be applied as markers to identify the different species in Veneridae. Liver and gill are better material for EST isozyme analysis and adductor muscle for MDH, because of the activity of enzyme is higher than other tissues.The electrophoretograms of isozymes (EST, MDH and LDH) from five different tissues (foot, mantle, adductor muscle, gill, liver) of M. mercenaria were analyzed. The results showed that there are some differences in the isozyme phenotypes of EST and MDH among five tissues, but no difference was found in LDH. The electrophoresis patterns of EST and MDH had a tissue-specific distribution. The physiological significance in relation to the tissue-specific distribution of the isozymes was discussed. And we found the gene of s-MDH and m-MDH in adductor muscle of M mercenaria.2. Fluorescent amplified fragment length polymorphism analysis of genetic diversity and species relationships of four clam species and population genetic structure of Meretrix meretrix in family Veneridae (Mollusca: Bivahia)Fluorescent amplified fragment length polymorphism (fAFLP) method was used to study the genetic diversity and species relationships of four species in family Veneridae. Digestion with enzymes EcoR I /Mse I and amplification with six E+3/M+3 primer... |
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