Studies On Morphology Identification And Ribosome And Mitochondrial DNA Polymorphism Of Ticks From Animals

Preliminary morphological identification and the DNA polymorphism analysis were conducted towards the animal ticks, with the purpose of clarifying the hereditary variation of intraspecific variation and the interspecifc differences. PCR amplification, cloning, sequencing and sequence analysis were used to discususs the sequence variation in internal transcribed spacers (ITS) of ribosomal DNA (rDNA) and cytochrome c oxidase subunitl (coxl) among the samples. Result shows:1.2 families,5 genuss and 8 species were identified, they are Boophilus decoloratus, Rhipicephalus pulchellus, Haemaphysalis flava, Boophilus microplus, Rhipicephalus sanguineus, Ornithodoros Lahorensis, Hyalomma and Amblyomma, and the paper describe the morphology of ticks from the hevivore zebra in Africa for the first time, the reason why there are differences among these ticks of this experiment and the evidence from the published literature is from different geography strain, different hemophagia stage, different hosts and different year; 2. PCR amplification of ITS-1 and ITS-2 were first operated on 6 ticks from 5 different animal species, and the sequences show some discrepancy together with the sequences of ticks recorded in the GenBank.3. It's the first time that the sequences of coxl of the 6 species were amplified by PCR, and the interspecies differences of basepairs was between 0%～21% through the discrepancy analysis with the tick coxl sequences recorded by the GenBank.The research testified that ITS-1, ITS-2 and coxl provide appropriate genetic markers for the genetic variation studies of animal ticks. The results of the present experiments contribute to understand the life-cycles, epidemiology and phylogenetic relationships of different ticks and also help to diagnose the diseases they spread.