Molecular Marker Analysis And Flora Gene CcLFY And Its Promoter Cloning Of Carya Cathayensis

Hickory, a dry fruit and oil tree in the world, is a special species in China. It mainly distributes in 29°～31°N and 118°～120°E of Tianmu mountain system matched between Zhejiang province and Anhui province. There are totally 70,000 farmers engaged in production and management of hickory, whose income occupied more than 70%. It provides new theoretic base and approach for fine variety breeding, establishes the base of molecular breeding for new species breeding of shorting flowering time and maturation ahead of time and is of great theoretic and practical important to improve this economic tree by conducting the molecular marker and cloning flowering gene and its promoter in hickory. The genetic relationship among Carya genus was analysized based on RAPD. The genetic diversity within populations and inter-populations from 5 Carya cathayensis and 3 C. dabieshanensis natural populations were also conducted. The genetic mutation of F1 generation hybridized between C. cathayensis and C. illinoensis was analyzed using AFLP. CcLFY, the flowering gene and its promoter were cloned and registered in GenBank by using routine molecular biology and anchoring PCR. The main results are summarized as follows:1. A total of 317 DNA fragments were amplified using 20 primers among 6 species in genus carya. It mean that average 15.58 DNA fragments per primer were amplified. 271 of these fragments were polymorphic, occupied 85.5%. The genetic relationship among 6 species in genus carya was conducted. The smallest is 0.2216 between Carya cathayensis and C.Hunanensis, which means the genetic relationship is the closest between them. The largest is 0.5440 between C.Hunanensis and C. illinoensis, which means the genetic relationship is the farthest between them.A total of 252 and 238 DNA fragments were amplified using 20 primers among 5 natural populations of C. cathayensis and 3 natural populations of C. cathayensis respectively. 681 (66.7%) and 162 fragments (68.1%) were polymorphic, respectively. Shannon index was 0.4102, 60.32% of which was from the inner-populations, 39.68% of...